激光诱导叶绿素荧光寿命的测量及其特性分析

提出了一种用于评估植物生长状况及环境监测的激光诱导叶绿素荧光寿命测量方法. 采用波长355 nm的激光作为光源激发叶绿素荧光, 由光电倍增管接收其荧光信号, 由于被测叶绿素荧光衰减函数与激光脉冲、仪器响应函数卷积在一起, 根据它们的特性, 运用时间分辨测量法分别测得叶绿素荧光及其背景信号, 并结合一种新型解卷积算法可分离出真实的叶绿素荧光衰减函数, 从而获取叶绿素的荧光寿命. 测试结果表明: 该方法能够实现叶绿素荧光寿命的高精度实时监测, 对不同叶绿素含量的溶液荧光寿命进行了测试, 证明叶绿素含量与其荧光寿命具有相关性, 并且拟合了叶绿素含量与荧光寿命的标定曲线. 关键词： 荧光寿命 激光诱导荧光 时间分辨测量法 叶绿素含量

Laser-induced chlorophyll fluorescence lifetime measurement and characteristic analysis

A laser-induced fluorescence lifetime measurement method is presented to evaluate living status for plant growth and the environmental monitoring. A 355 nm laser is used as excitation source for exciting chlorophyll fluorescence, and the fluorescence signals are received by a photomultiplier. Because the measured signal is the convolution of the reliable fluorescence decay signals, laser pulse and instrument response function, according to their characteristics, the time-resolved measurement method is used to estimate the chlorophyll fluorescence and background signals separately; the real fluorescence decay signals are separated by combining a novel deconvolution method, and the chlorophyll fluorescence lifetime can be retrieved. Experiment shows that the method proves to be a high accuracy and real-time monitoring chlorophyll fluorescence lifetime technique; and the chlorophyll solution fluorescence lifetime is measured for different concentrations. The result can prove that the chlorophyll concentration is related to its fluorescence lifetime, and the calibration curves of chlorophyll concentration and fluorescence lifetime is fitted.
Keywords： fluorescence lifetime laser-induced fluorescence time-resolved measurement chlorophyll concentration