天门冬组培快繁体系研究

目的 建立天门冬组培快速繁殖体系,保护和合理利用天门冬资源.方法 采用不同基本培养基和不同种类及质量浓度的植物生长调节剂对天门冬不同外植体进行愈伤组织诱导、丛生芽诱导及生根培养研究.结果 愈伤组织诱导的最适培养基为1/2 MS+1.0 mg/L 6-BA+0.5 mg/L NAA,pH 5.8;丛生芽诱导的适宜培养基为MS+ 1.5 mg/L 6-BA+0.5 mg/L LAA,pH 5.8;生根的适宜培养基为1/2 MS+0.5 mg/L IBA+0.5 mg/L IAA,其中培养基中铁盐的质量浓度降低到6.95 mg/L,蔗糖的质量浓度降低至20 g/L,pH值为5.8.结论 天门冬不同外植体均可诱导出愈伤组织,其中嫩芽诱导愈伤率最高,为天门冬最适组织培养的外植体;利用组织培养技术能够实现天门冬快速繁殖,为其人工扩大栽培奠定了良好基础.

Rapid propagation system for tissue culture of Asparagus cochinchinensis

Objective Establishing the rapid propagation for plant tissue culture of Asparagus cochinchinensis in order to rationally protect and utilize the medicinal plant resources of A.cochinchinensis.Methods Different media and plant growth regulators at different concentration were used to A.cochinchinensis explants for callus induction,cluster buds induction,and rooting.Results The best medium for callus induction was 1/2 MS + 1.0 mg/L 6-BA + 0.5 mg/L NAA with pH 5.8;The optimal medium for cluster buds induction was MS + 1.5 mg/L 6-BA + 0.5 mg/L IAA with pH 5.8;The optimal medium for rooting was 1/2 MS + 0.5 mg/L IBA + 0.5 mg/L IAA with pH 5.8(iron salt in MS medium reduced to 6.95 mg/L and sucrose decreased to 20 g/L).Conclusion Different explants could be induced for tissue culture and tender bud is the optimal tissue culture explants with the highest induction rate.An effective approach for rapid propagation has been provided,which could promote the artificial cultivation of A.cochinchinensis.