青蒿琥酯对口腔鳞癌Tca8113细胞及其移植瘤小鼠的抗肿瘤作用研究

目的 观察青蒿琥酯对口腔鳞癌Tca8113细胞增殖和凋亡的影响,以及对Tca8113细胞移植瘤小鼠肿瘤生长的影响.方法 用不同质量浓度的青蒿琥酯处理Tca8113细胞,HE染色观察细胞形态的改变;MTT法检测细胞增殖的情况;流式细胞仪检测细胞凋亡和细胞周期的变化.用口腔鳞癌Tca8113细胞建立口腔鳞癌淋巴道转移模型,ip给予移植瘤小鼠青蒿琥酯200 mg/(kg·d),连续给药10d,观察青蒿琥酯对肿瘤生长抑制作用,以5-氟尿嘧啶(5-FU)作为阳性对照.结果 青蒿琥酯作用Tca8113细胞后,形态学观察可见细胞凋亡现象,部分细胞明显肿胀、变圆、变大,溶解成碎片,甚至死亡;细胞增殖受到抑制且呈时间、剂量相关性;青蒿琥酯能有效诱导Tca8113细胞凋亡并呈时间、剂量相关性,还能将Tca8113细胞阻滞在G0/G1期.体内实验显示,青蒿琥酯对Tca8113细胞移植瘤具有明显的抑制作用,抑瘤率为41.18％.结论 青蒿琥酯体内及体外对口腔鳞癌Tca8113细胞均有抑制作用,其机制可能与青蒿琥酯诱导细胞凋亡或改变细胞周期的分布有关.

Antitumor effect of artesunate on oral squamous carcinoma Tca8113 cells and their transplanted tumor

Objective To study the growth inhibition of artesunate(ART) on Tca8113 cells in vitro and on their transplanted tumor in vivo.Methods Tca8113 cells were stimulated by different concentration of ART,cell morphology was observed by HE staining,cell proliferation was tested by MTT assay,and cell apoptosis and cell cycle were tested by flow cytometry.At the same time,lymphatic metastasis model of oral squamous carcinoma was established using Tca8113 cell line,the transplanted tumor mice were ip administrated with ART in a dose of 200 mg/(kg.d) for 10 d,and the inhibition of ART on tumor cells was observed,with 5-FU as positive control group.Results Cell apoptosis could be observed after ART stimulating,and some cells swelled,were rounded,largened,dissolved into pieces,and even died.ART could inhibit the proliferation and efficiently induce the apoptosis of Tca8113 cells,and the effects were in a time-and dose-dependent manner.Cell cycle was arrested at G0and G1phase,ART could obviously depress the growth of transplanted tumor,and the inhibitory ratio was 41.18%.Conclusion ART could inhibit the proliferation of Tca8113 cells both in vitro and in vivo,and the mechanism is possibly by inducing the apoptosis or changing the cell cycle distribution.