硼中子俘获治疗对裸鼠脏器影响的实验研究

目的采用动物实验方法研究硼中子俘获疗法(BNCT)对于正常脏器的损伤作用,从而为其安全性研究提供依据。方法实验动物裸小鼠随机分成单纯照射中子组(Ⅰ组),单纯给药组(Ⅱ组),BNCT 1、2、3组(Ⅲ、Ⅳ、Ⅴ组)。其中BNCT 1组为注射硼携带剂BPA(300 mg/kg,B.W.)2.5 h后接受超热中子束满功率照射10 min,BNCT 2组为注射硼携带剂BPA(500 mg/kg,B.W.)2.5 h后接受超热中子束满功率照射10 min,BNCT 3组为注射硼携带剂BPA(300 mg/kg,B.W.)2.5 h后接受超热中子束满功率照射15 min。实验完成后2周处理动物,将各组动物脏器心、肺、肝、脾、肾、膀胱切取后HE染色,病理切片,两名经验丰富的病理科医师观察脏器淤血,水肿,坏死,增生情况。结果将各组数据统计学处理采用等级计数资料的Ridit分析。结果显示各组心、肺、脾、肾、膀胱损伤及肝细胞坏死及非典型增生差异无统计学意义,肝组织淤血、水样变性实验组损伤小于对照组,差异有统计学意义。结论 BNCT对裸鼠脏器损伤小,是一项安全的治疗方法,为进一步用BNCT治疗肿瘤提供了依据。

The effect of boron neutron capture therapy on internal organs of nude mice

Objective The effect of boron neutron capture therapy（BNCT） on normal organs was examined in nude mice.Methords Thirty mice were randomly allocated into five groups： control group without BPA（group I）,control group without radiation（group II）,BNCT-1,BNCT-2 and BNCT-3（Ⅲ,Ⅳ and Ⅴ） groups.The histological examination of internal organs including heart,lung,liver,spleen,kidney and bladder were taken two weeks later.Results The results indicated that BNCT did not increase the possibility of necrosis and atypical hyperplasia in internal organs including heart,lung,liver,spleen,kidney and bladder in comparison with those of control group,and the occurrence of congestion and hydropic degeneration in liver tissues was less than those of control group.Conclusion The BNCT protocols indicated that BNCT at selected dosage does not impair the normal function of internal organs.