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大白菜抗根肿病的抗源筛选和分子标记鉴定
引用本文:朱明钊,张淑江,张慧,章时蕃,李菲,王晓武,武剑,李国亮,魏云晓,岳丽昕,孙日飞.大白菜抗根肿病的抗源筛选和分子标记鉴定[J].中国蔬菜,2018,1(3):40-45.
作者姓名:朱明钊  张淑江  张慧  章时蕃  李菲  王晓武  武剑  李国亮  魏云晓  岳丽昕  孙日飞
作者单位:中国农业科学院蔬菜花卉研究所,北京 100081
基金项目:国家重点研发计划项目(2017YFD0101802),农业部园艺作物生物学与种质创制重点实验室项目
摘    要:以3种根肿菌M01、M02、M03为病原菌菌源,对24份大白菜材料进行接种鉴定和分子标记鉴定,旨在获得优良的抗源材料并明确材料中含有的抗病基因。结果表明:24份大白菜材料中共有10份抗病材料,5份材料对3种根肿菌都表现抗病:其中CR004和CR007含有根肿病抗病基因CRk,CR013含有抗病基因CRa、CRbkato、CRk和Crr3,CR015和CR016含有抗病基因CRa、CRbkato和Crr3;3份材料对根肿菌M01和M02表现抗病:CR012含有抗病基因CRa和CRbkato,CR014和CR018含有抗病基因CRa、CRbkato和CRk;抗根肿菌M01的材料CR021含有抗病基因Crr2和CRk;抗根肿菌M02的材料CR023含有抗病基因CRc和CRk。

关 键 词:大白菜  根肿病  抗源筛选  分子标记

Anti-sources Screening and Molecular Marker Identification of Chinese Cabbage Against Clubroot
ZHU Ming-zhao,ZHANG Shu-jiang,ZHANG Hui,ZHANG Shi-fan,LI Fei,WANG Xiao-wu,WU Jian,LI Guo-liang,WEI Yun-xiao,YUE Li-xin,SUN Ri-fei.Anti-sources Screening and Molecular Marker Identification of Chinese Cabbage Against Clubroot[J].China Vegetables,2018,1(3):40-45.
Authors:ZHU Ming-zhao  ZHANG Shu-jiang  ZHANG Hui  ZHANG Shi-fan  LI Fei  WANG Xiao-wu  WU Jian  LI Guo-liang  WEI Yun-xiao  YUE Li-xin  SUN Ri-fei
Affiliation:Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Abstract:Twenty-four Chinese cabbage(Brassica rapa L. ssp. chinensis)materials were inoculated with 3 kinds of Plasmodiophora brassicae M01,M02 and M03,and all the lines were identified by molecular markers in order to obtain excellent anti-sources and to confirm all materials containing disease resistant genes. The results showed that there were 10 out of 24 materials were resistant to disease. Among them 5 were resistant to 3 kinds of Plasmodiophora. Lines CR004 and CR007 contained the disease resistant gene CRk,CR013 containing disease resistant genes CRa,CRbkato,CRk and Crr3. CR015 and CR016 contained the disease resistant genes CRa,CRbkato and Crr3. All 3 materials showed resistance to M01 and M02. Line CR012 contained disease resistant genes CRa and CRbkato. CR014 and CR018 contained disease resistant genes CRa,CRbkato and CRk. The CR021 line contained disease resistant genes Crr2 and CRk. The CR023 line contained disease resistant genes CRc and CRk showed resistance to M02.
Keywords:Brassica rapa  Clubroot  Anti-source screening  Molecular marker  
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