北京地区儿童ARI的鼻咽分泌物的病毒免疫荧光检测结果分析

目的 分析1997年3月－1999年3月两年间北京地区儿童急性呼吸道感染（ARI）的鼻咽分泌物病毒免疫荧光学检测结果。方法 选择1997年3月－1999年3月因急性呼吸道感染而在北京市儿童医院住院治疗的患儿，取其鼻咽分泌物做免疫荧光检测筛查七种呼吸道病毒抗原，阳性病例入选，分析其病历资料。结果 所有794例病人送检标本共796份，阳性228份（28．6％），其中RSV196例（24．6％）、IA20(2．5％）、IB1例（0．12％）、ADV4例（0．5％）、PIV3 2例（0．25％）、RSV和PIV3 2例（0．25％），RSV和IA3例 （0．38％）。RSV 和IA感染呈季节性分布。RSV组和IA组病人之间喘息症状并无差异。小年龄组病人与大年龄儿相当，呼吸快、更多存在低氧血症和二氧化碳潴留，病情重、吸氧者多。结论 北京地区两年以来儿童呼吸道感染的病毒学病因可能为呼吸道合胞病毒（RSV）、流感病毒（IA）、腺病毒（ADV）和副流感病毒3型（PIV3），以RSV为主。RSV和IA有一定的季节变化。

An Analysis of Detecting Results on Viral Pathogens of ARI from NPS by IFA among Children in Beijing.

Objective To investigate the viral pathogens of children's ARI in Beijing area from March 1997 to March 1999. Methods To choose the patients who were admitted to Beijing Children's Hospital from March,1997 to March,1999.They had symptoms of ARI and had the nasopharyngeal secretions(NPS) examined by immunofluorescence assay(IFA).The records of the patients whose IFA results were positive were carefully reviewed and analyzed by SPSS software. Results Among all the 794 patients (796 samples),there were 228 (28.6%) positive results.196(24.6%) positive samples were respiratory syncytial virus(RSV),20(2.5%) were influenza virus type A(IA),1(0.12%) was influenza virus type B(IB),4(0.5%) were adenovirus(ADV) and 2 (0.25%) samples were positive of Parainfluenza virus (PIV 3).Also there were 2 cases(0.25%),which were positive of RSV and PIV 3 together,and 3(0.38%) were RSV and IA positive simultaneously.RSV and IA had a seasonal trend.There was no significant difference of wheezing symptom between RSV positive patients and IA positive patients.Compared with the elders,the younger patients had much faster respiratory rates,more hypoxemia and hypercapnia.They were much severer than the elders and needed more oxygen therapies. Conclusions RSV,IA,ADV and PIV 3 were the common viral detection from NPS by IFA, causing respiratory infections during these two years in Beijing,among which RSV was most predominant one.RSV and IA had a seasonal trend.