| 氢气预处理对乳鼠海马神经元细胞缺氧/复氧损伤后细胞活力的影响 |
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| 引用本文： | 卢欣. 氢气预处理对乳鼠海马神经元细胞缺氧/复氧损伤后细胞活力的影响[J]. 中国实用神经疾病杂志, 2012, 15(11): 10-11 |
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| 作者姓名： | 卢欣 |
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| 作者单位： | 郑州大学基础医学院,郑州,450052 |
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| 摘 要： | 目的探讨氢气对乳鼠海马神经元细胞缺氧/复氧损伤后细胞活力的影响。方法原代培养的SD乳鼠海马神经元细胞,随机分成对照组,缺氧/复氧组和氢气预处理组。对照组常规培养;缺氧/复氧组在100%N2中培养15min后复氧30min;氢气预处理组在2%H2+98%N2中培养15min后复氧30min。采用MTT法检测乳鼠海马神经元细胞活力。结果氢气预处理能够增强细胞活力(P<0.05)。结论氢气预处理对海马神经元细胞缺氧/复氧损伤具有保护作用,可能与提高细胞活力有关。
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| 关 键 词： | 海马神经元细胞 氢气 缺氧/复氧损伤 |
Effect of preconditioning with hydrogen on hypoxia/reoxygenation injury of neonatal rat hippocampal neurons' cell viability |
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| Lu Xin. Effect of preconditioning with hydrogen on hypoxia/reoxygenation injury of neonatal rat hippocampal neurons' cell viability[J]. Chinese Journal of Practical Neruous Diseases, 2012, 15(11): 10-11 |
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| Authors: | Lu Xin |
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| Affiliation: | Lu Xin.Basic Medical College,Zhengzhou University,Zhengzhou 450052,China |
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| Abstract: | Objective To explore the effect of the hydrgeon on hypoxia/reoxygenation injury of cultured neonatal rat hippocampal neurons’cell viability.Methods Primary cultured hippocampal neurons of SD rats were randomly assigned to control group,hypoxic reoxygenation(H/R)group and hydrogen preconditioning group.Except for the control group,the other groups were subjected to 15minutes of 100% N2or 2% H2+98% N2respectively for pretreating of hypoxia,and 30minutes of reoxygen.The cell viability was measured by using MTT way.Results Hydrogen preconditioning increased the cell viability in the injured hippocampal neurons(P<0.05).Conclusion Hydrgeon has a protective effect on hypoxia/reoxygenation injury of hippocampal neurons.The effect may be involved in enhancing the cell viability. |
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| Keywords: | Hippocampal neurons Hydrgeon Hypoxia/reoxygenation injury |
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