Abstract: | Luminescent carbon‐based nanomaterials hold great promise due to their stable photo‐physical behaviour, biocompatibility and lower toxicity. This work involves economic and facile one‐pot green synthesis of water‐soluble nanostructures from lemon grass (LGNS) [Cymbopogon citratus (DC) Stapf] as carbon source. High‐resolution transmission electron microscopy confirmed the formation of LGNS with lattice spacing of 0.23 nm matching low‐dimensional graphitic structures. The strong absorption exhibited at 278 nm could be attributed to л‐states of sp2 /sp3 hybridisation in carbon nanostructures. Fluorescence spectroscopy of LGNS exhibited strong excitation‐dependent emission properties over a broad range of wavelengths from 300 to 600 nm. Quantitatively, these LGNS were estimated to have quantum yield of 23.3%. Biomass derived LGNS could be potentially exploited for wide variety of applications like bioimaging, up‐conversion, drug delivery and optoelectronic devices. To this extent, synthesised LGNS were used to image yeast cells via multicolour/multi‐excitation fluorescence imaging.Inspec keywords: fluorescence, carbon, nanofabrication, photoluminescence, toxicology, transmission electron microscopy, cellular biophysics, biomedical optical imaging, nanomedicine, biomedical materials, microorganisms, liquid phase depositionOther keywords: one‐pot hydrothermal synthesis, biocompatible fluorescent carbon dots, lemon grass herb, luminescent carbon‐based nanomaterials, stable photophysical behaviour, toxicity, water‐soluble nanostructures, carbon source, high‐resolution transmission electron microscopy, low‐dimensional graphitic structures, hybridisation, carbon nanostructures, fluorescence spectroscopy, excitation‐dependent emission properties, biomass derived LGNS, bioimaging, drug delivery, optoelectronic devices, yeast cell image, multicolour‐multiexcitation fluorescence imaging, C |