远隔缺血后适应减轻大脑缺血再灌注损伤

目的:探讨远隔缺血后适应对局灶性脑缺血再灌注损伤后大脑梗塞面积及血脑屏障(blood brainbarrier,BBB)通透性功能障碍的保护性作用及其程度。方法:制备大鼠缺血再灌注损伤模型,建立远隔缺血后适应。应用TTC法评估脑梗死面积,采用干湿重法计算脑含水量评估脑水肿、应用伊文思蓝(Evans Blue,EB)观察再灌注后BBB通透性变化。结果:大鼠BBB通透性缺血后适应24 h组与缺血再灌24 h组相比明显减轻(P<0.05),缺血后适应48 h组与缺血再灌注48 h组相比明显减轻(P<0.05);TTC梗塞面积缺血后适应24 h组与缺血再灌注24 h组相比明显减轻(P<0.0001),缺血后适应48 h组与缺血再灌注48 h组比明显减轻(P<0.0001);缺血后适应组比缺血再灌注组脑含水量明显减少(P<0.01)。结论:缺血后适应能够保护缺血再灌注后血脑屏障的破坏,减轻梗塞面积和脑水肿。

Effect of remote ischemic post-conditioning on reducing brain damage in experimental ischemia/reperfusion

Objective:To determine the protective effect of remote ischemic post-conditioning on BBB and cerebral infarction in ischemia/reperfusion injury.Methods:Ischemia/reperfusion injury models of rats and remote ischemic post-conditioning were established.Evans Blue(EB) dye extravasations can judge the damage of the BBB.Cerebral infarction volumes were quantified by TTC staining and dry/wet weight method was used to determine the brain water content.Results:Both BBB permeability and TTC cerebral infarct area of rats in ischemic postconditioning groups(24 h,48 h) had significant decrease compared to those in ischemia reperfusion groups(24 h,48 h) in turn(P<0.05 for BBB permeability,P<0.0001 for TTC cerebral infarct area).Brain edema relieved significantly in ischemic postconditioning group compared to ischemic reperfusion group(P<0.01).Conclusion:These results provide evidence that ischemic post-conditioning can protect blood brain barrier and reduce brain edema and brain infarct volume in experimental ischemic stroke.