In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana |
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Authors: | Zhelmy Martí n-Quintal,Rosa Moo-Puc,Francisco Gonzá lez-Salazar,Manuel J. Chan-Bacab,Luis W. Torres-Tapia,Sergio R. Peraza-Sá nchez |
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Affiliation: | 1. Centro de Investigación Científica de Yucatán, Calle 43 #130, Col. Chuburná de Hidalgo, Mérida, Yucatán 97200 (CICY), Mexico;2. Unidad de Investigación Médica Yucatán, Unidad Médica de Alta Especialidad, Centro Médico Ignacio García Téllez, Instituto Mexicano del Seguro Social, Calle 41 #439, Col. Industrial, Mérida, Yucatán 97150, Mexico;3. División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, IMSS, Monterrey, Nuevo León 64720, Mexico;4. Departamento de Microbiología Ambiental y Biotecnología, Universidad Autónoma de Campeche, Av. Agustín Melgar s/n, Campeche 24030, Mexico |
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Abstract: | Ethnopharmacological relevanceTridax procumbens is an active herb against leishmaniasis.Aim of the studyLeishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World.Materials and methodsExtracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT.ResultsWe demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC50) of 3 μg/ml, while oxylipin 1 exhibited the highest inhibition at IC50 = 0.478 μg/ml.ConclusionsIn this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells. |
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Keywords: | Tridax procumbens Leishmania mexicana Promastigotes (3S)-16,17-Didehydrofalcarinol Oxylipin Cytotoxicity |
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