大豆及其制品中转基因成分的二重PCR检测

为了建立能同时检测多种转基因成分的二重PCR检测方法,以转基因大豆(RoundupReady品种)为实验材料,优化了二重PCR的反应体系和反应条件,包括引物浓度、TaqDNA酶用量和退火温度等;比较了二重PCR和单一PCR的灵敏度和检测含量。结果表明:二重PCR的灵敏度和检测含量与单一PCR的相当;用建立的二重PCR方法从大豆、豆腐、豆粕和油炸豆腐中筛选出含转基因成分的阳性样品;二重PCR与单一PCR相比,具有节约试剂、省时等特点,在转基因产品检测上具有很好的推广价值。

Detection of Genetically Modified Components in Soybean and Its Products by Duplex PCR

In order to developing duplex PCR method which could detect synchronously several genetically modified components, the reaction system and condition of the duplex PCR were optimized using transgenic Roundup Ready soybean as experimental material, including the concentration of primers, units of Taq DNA polymase and anneal temperature. The sensitivity and detection content of duplex PCR were compared with those of single PCR. The results showed that sensitivity and detection content of duplex PCR optimized were as the same as those of single PCR. The positive samples with genetically modified components were screened successfully from soybean, bean curb, soya residue and fried bean curb by the duplex PCR developed. The reagent and time needed for duplex PCR were less than those of sinlge PCR. Therefore,the duplex PCR might be popularized to detect genetically modified products.