C57BL/6J小鼠PASG基因敲除后多器官异常表型初探

目的探寻PASG基因敲除对脑等多器官发育的影响，为研究胶质瘤发生的分子病因奠定基础。方法采用经典的基因敲除法选择性缺失小鼠PASG基因第10～l2外显子，并以组织学及Western bolt法检测基因敲除鼠的中枢神经细胞形态和基因改变。结果PASG基因敲除小鼠显示出脑、肺、肾和骨等器官一系列表型变化，尤其是脑发育延缓，海马区组织结构异常，及相关基因表达水平改变等。结论PASG是调控细胞增殖发育相关基因，它的缺失可导致多器官功能异常，尤其是脑海马区细胞的变化与神经干细胞关系密切，可为进一步研究胶质瘤发生的分子病因提供线索。

Primary Study on Phenotype of Multi-organs Abnormality Resulting from PASG Gene Knock-out in C57BL/6J Mice

Objective To explore effects of PASG knockout on brain development and lay a foundation for research on molecular etiology of glioma. Methods No. 10-12 exons of murine PASG gene were selectively deleted with classical knockout method. Histological analysis and western blot assay were employed to examine CNS cell morphology and gene expression in mutant mice. Results PASG mutant mice displayed functional abnormality in multi-organs (brain, lung, kidney, bone and so on), especially neurological development retardation, histological changes in hippocampal region and expression changes of related genes. Conclusions PASG is essential for regulating embryo development and cell proliferation. The disruption of PASG caused abnormality structurally and functionally in organs of mice. Moreover, the close relationship between neural stem cells and cells in hippocampus provided a new access to molecular mechanism of glioma formation.