益气消积方含药血清对人胃癌NKN-28细胞株的作用

目的:研究益气消积方含药血清对人胃癌NKN-28细胞株的作用。方法:以SD大鼠为实验血清的供体,将大鼠血清灭活,以灭活前给药与否及给药剂量分为高、中、低剂量组及空白血清组,以细胞抑制率为观察指标,对实验结果进行分析。结果:4组大鼠血清对NKN-28细胞株生长均有抑制作用,且对NKN-28细胞株生长的抑制作用具有一定的浓度依赖性;实验组各浓度组间差异有统计学意义(P<0.01)。结论:益气消积方含药血清可明显抑制NKN-28细胞株的生长,抑制作用呈一定的浓度依赖性。

Effects of serum containing Yiqi Xiaoji Recipe on cell line NKN-28 of gastric cancer

Objective: To explore the effects of serum containing Yiqi Xiaoji Recipe (YQXJR), a compound traditional Chinese herbal medicine for benefiting qi and removing stagnation, on cell line NKN-28 of gastric cancer. Methods: The experimental SD rats were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into high-dose, medium-dose, low-dose and blank serum groups based on whether the rats were given YQXJR and administration dosage. The inhibition rate was regarded as the observational index.Results: The four groups of serum all had inhibitory effect on the growth of NKN-28 cells depending on the drug concentration. And there were significant differences among the experimental groups. High-dose, medium-dose and low-dose concentrations of serum all could inhibit the growth of NKN-28 cells with positive relations with the concentration and function time.Conclusion: YQXJR serum can inhibit the growth of NKN-28 cells depending on the drug concentration and function time.