| 放线菌酮对米根霉积累L-苹果酸代谢途径的调控作用 |
| |
| 引用本文： | 何皓,李霜,徐晴,张凯,黄和.放线菌酮对米根霉积累L-苹果酸代谢途径的调控作用[J].过程工程学报,2009,9(1):153-156. |
| |
| 作者姓名： | 何皓 李霜 徐晴 张凯 黄和 |
| |
| 作者单位： | 南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,江苏,南京,210009 |
| |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展规划(973计划)，国家高技术研究发展计划(863计划)，基金，江苏省自然科学基金 |
| |
| 摘 要： | 实验考察了富马酸酶活抑制剂放线菌酮对米根霉发酵产L-苹果酸和富马酸的影响. 结果表明，发酵16 h加入浓度15 mg/L的放线菌酮使米根霉富马酸酶胞质同功酶比活力峰值降低48.7%，而苹果酸脱氢酶胞质同功酶活力却未有明显改变，生成的富马酸比对照样减少37.1%，而L-苹果酸积累量提高54.6%，达到21.7 g/L. 表明通过对米根霉富马酸酶胞质同功酶的抑制作用能减弱米根霉积累富马酸代谢途径中由L-苹果酸向富马酸的转化，从而促进米根霉积累L-苹果酸.
|
| 关 键 词： | L-苹果酸 米根霉 放线菌酮 富马酸酶 富马酸 |
| 收稿时间： | 2008-10-13 |
| 修稿时间： | 2008-12-2 |
Effect of Cycloheximide on Regulation of Metabolic Pathway for L-Malic Acid Accumulation by Rhizopus oryzae |
| |
| HE Hao,LI Shuang,XU Qing,ZHANG Kai,HUANG He.Effect of Cycloheximide on Regulation of Metabolic Pathway for L-Malic Acid Accumulation by Rhizopus oryzae[J].Chinese Journal of Process Engineering,2009,9(1):153-156. |
| |
| Authors: | HE Hao LI Shuang XU Qing ZHANG Kai HUANG He |
| |
| Affiliation: | State Key Lab.Materials-oriented Chem.Eng.;Col.Life Sci.Pharmacy;Nanjing Univ.Technol.;Nanjing;Jiangsu 210009;China |
| |
| Abstract: | The effects of fumarase inhibitor cycloheximide on fermentation of L-malic acid and fumaric acid by Rhizopus oryzae ME-M15 were investigated.The results showed that the addition of cycloheximide(15 mg/L) during the acid production stage after 16 h incubation greatly affected the metabolism of Rhizopus oryzae ME-M15.The activity of cytosolic isoenzyme of fumarase was decreased markedly by 48.7%, but the activity of NAD-malate dehydrogenase in the cytosol fraction did not change significantly in the presence ... |
| |
| Keywords: | L-malic acid Rhizopus oryzae cycloheximide fumarase fumaric acid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《过程工程学报》浏览原始摘要信息 |
|
点击此处可从《过程工程学报》下载全文 |
