MicroRNA-590和microRNA-196b在儿童IgA肾病诊断中的价值

目的 探讨IgA肾病患儿血清microRNA-590(miR-590)、microRNA-196b(miR-196b)水平的变化及其临床价值.方法 选取2017年9月—2019年5月于青海省妇女儿童医院肾活检患儿118例,83例确诊为IgA肾病患儿,35例为其他肾小球疾病患儿,另选取同期该院体检健康儿童42例为对照组....

Values of microRNA-590 and microRNA-196b in diagnosis of IgA nephropathy children

Objective To investigate the changes and clinical values of serum microRNA-590 (miR-590) and microRNA-196b (miR-196b) levels in children with IgA nephropathy.Methods From September 2017 to May 2019, the children underwent renal biopsy in our hospital were collected, among them, 83 cases were diagnosed as IgA nephropathy, 35 children with other glomerular diseases, and another 36 healthy children without kidney disease were selected as the control group. The expression levels of miR-590 and miR-196b were detected by qRT-PCR. According to Lee''s classification, the pathological classification of IgA nephropathy was carried out. The diagnostic values of serum miR-590, miR-196b and their combined detection in IgA nephropathy were evaluated by drawing the receiver operation characteristic curve (ROC).Results Compared with the control group and non-IgA nephropathy group, the levels of creatinine, 24-hour album inuria, and serum IgA in IgA nephropathy group increased (P < 0.05), while the level of albumin decreased (P < 0.05). There was no significant difference in the levels of miR-590 and miR-196b between the non-IgA nephropathy group and the control group (P > 0.05), the expression level of miR-590 decreased in IgA nephropathy group (P < 0.05), and decreased with the aggravation of the disease, the difference between the any two groups was statistically significant (P < 0.05); the expression level of miR-196b increased (P < 0.05), and increased with the aggravation of the disease, and the difference between the any two groups was statistically significant (P < 0.05); ROC curve showed that the area under the curve of miR-590, miR-196b, and combinationin diagnosing IgA nephropathy was 0.873 (sensitivity was 84.1%, and specificity was 82.6%), 0.785 (sensitivity was 90.7%, and specificity was 64.8%), 0.904 (sensitivity was 88.6%, and specificity was 87.0%), compared with the level of miR-196b in the diagnosis of IgA nephropathy, the diagnosis effect of combined detection of miR-590 and miR-196b was higher (P < 0.05).Conclusions The expression of miR-590 in serum of children with IgA nephropathy is low, and the expression of miR-196b in serum is high, the combination of the two has high diagnostic value for IgA nephropathy.