产地加工与饮片炮制一体化对苦参饮片主要功效的影响

目的:比较产地加工与炮制一体化加工与传统方式加工苦参饮片的抗炎与解热作用。方法:采用二甲苯致小鼠耳肿胀试验,观察2种苦参饮片水提物的抗炎消肿作用;采用酵母致热大鼠模型,比较2种苦参饮片水提物的解热作用。结果:与空白组比较,2种苦参饮片水提物均可降低小鼠耳肿胀度,但以一体化加工苦参饮片的抗炎消肿作用更为显著,各剂量组抑制率均40%。一体化加工苦参饮片3个剂量均能降低发热大鼠体温,且作用时间可持续4 h,解热作用优于传统加工的苦参饮片。一体化加工苦参饮片中槐果碱、苦参碱、氧化槐果碱、槐定碱及氧化苦参碱等5种主要有效成分的含量均高于传统方式加工的苦参饮片,其总量约为传统苦参饮片的1.3倍。结论:一体化加工与传统加工方式生产的苦参饮片在抗炎及解热等主要功效方面具有较好的相似性,而且一体化加工方式从源头规范了苦参的饮片生产,在确保饮片质量的前提下缩短了生产周期,是一种值得推广应用的中药饮片生产模式。

Effect of Integration of Habitat Processing and Pieces Processing on Main Efficiency of Sophorae Flavescentis Radix Products

Objective: To compare anti-inflammatory and antipyretic effect of Sophorae Flavescentis Radix pieces between integrated production process and traditional processing method. Method: Anti-inflammatory and detumescence effects of water extracts of two Sophorae Flavescentis Radix pieces was observed by xylene-induced ear edema in mice.Antipyretic effect of water extracts of two kinds of Sophorae Flavescentis Radix pieces was compared in febrile rats induced by yeast. Result: Compared with the blank group,water extracts of two kinds of Sophorae Flavescentis Radix pieces could reduce the ear edema of mice,but the anti-inflammatory and detumescence effects of the integrated processed pieces was more significant,the inhibition rates of its each dose group were more than 40%.Antipyretic experiment showed that all the three doses of integrated processed pieces could decrease the body temperature of fever rats for 4 hours,and the antipyretic effect was superior to the traditional processed pieces.The contents of five main active ingredients in integrated processed pieces,such as sophocarpine,matrine,N-oxysophocarpine,sophoridine and oxymatrine,were higher than these in traditional processed pieces,their total amount was about 1.3 times to the traditional processed pieces. Conclusion: There has good similarity in the main effect of anti-inflammatory and antipyretic between integrated processed and traditional processed pieces,the integration processing method will standardize production process from the source,and it can shorten the production cycle under the premise of ensuring the quality of pieces.