| 大绒鼠(Eothenomy miletus)微卫星多态性引物的筛选 |
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| 引用本文： | 朱万龙,刘春燕,张麟,王政昆. 大绒鼠(Eothenomy miletus)微卫星多态性引物的筛选[J]. 云南师范大学学报(自然科学版), 2013, 33(2): 63-69 |
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| 作者姓名： | 朱万龙 刘春燕 张麟 王政昆 |
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| 作者单位： | 云南师范大学生命科学学院,云南昆明,650500 |
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| 基金项目： | 国家自然科学基金资助项目，云南省应用基础研究面上资助项目 |
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| 摘 要： | 大绒鼠是横断山区的固有种。利用大绒鼠40个个体设计出12对微卫星引物,其有效等位基因(Na)2～5个,观测杂合度(HO)、期望杂合度(HE)及多态信息含量(PIC)分别为0.111to 0.465,0.457to 0.707,0.407 3to 0.674 1.筛选出的12对引物中Emj 2～43和Emj 2～54属于高度多态性座位,其余10对均为中度多态性座位,这些微卫星引物的筛选可用于今后更进一步的种群研究。
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| 关 键 词： | 微卫星 大绒鼠 种群结构 |
Isolation and Polymorphic Loci of Microsatellite in Eothenomys Miletus (Microtinae Eothenomys) |
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| ZHU Wan-long , LIU Chun-yan , ZHANG Lin , WANG Zheng-kun. Isolation and Polymorphic Loci of Microsatellite in Eothenomys Miletus (Microtinae Eothenomys)[J]. Journal of Yunnan Normal University (Natural Sciences Edition), 2013, 33(2): 63-69 |
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| Authors: | ZHU Wan-long LIU Chun-yan ZHANG Lin WANG Zheng-kun |
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| Affiliation: | (School of Life Science,Yunnan Normal University,Kunming 650500,China) |
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| Abstract: | The Oriental vole Eothenomys miletus（Microtinae：Eothenomys）is an endemic species distributed in Hengduan mountain region.In this study,twelve microsatellite loci were isolated from this species.Analysis of 40 individuals showed that the number of alleles ranged from two to five with observed heterozygosity ranged from 0.111 to 0.465,expected heterozygosity ranged from 0.457 to 0.707,and polymorphic information content ranged from 0.407 3 to 0.674 1.These microsatellite loci will be of practical use for population structure,mating system and inbreeding studies in E.miletus. |
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| Keywords: | Microsatellite marker Eothenomys miletus Population structure |
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