模块化共培养技术在代谢工程领域的应用及研究进展

利用代谢工程方法生物合成目标产物时,需要为设计好的生物合成途径筛选一个合适的微生物宿主,关于此方面的研究在过去十几年中已取得了巨大的成果。随着代谢工程领域的发展,有很多先进的方法被不断地建立与应用,这就对微生物宿主细胞提出了更高的要求。近期的许多科学研究应用共培养体系来减轻细胞压力,从而提高目标产物的产量。模块化共培养体系由多种工程生物菌株组成,可对生物合成进行模块化构建。这种新兴的模块化共培养的工程方法可以广泛地应用于代谢工程领域,具有很高的应用价值。本文总结并讨论了模块化共培养工程方法的优势以及主要面临的挑战,同时对模块化共培养工程的研究进展进行了概述。

Application and progress of modular co-culture engineering in metabolic engineering field

Metabolic engineering is used to biosynthesize target products and it is necessary to screen a suitable microbial host for the designed biosynthetic pathway. The research on this strategy has made great achievements in the past decade. With the development of metabolic engineering, more advanced metabolic engineering methods have been established and applied, which has higher demand for biosynthetic microorganism host cells. In order to enhance the production of target products, many scientific studies have applied co-culture engineering to relieve the pressure of strain. The modular co-culture engineering is composed of a variety of engineering biological strains, which can be modular construction by biosynthesis. Modular co-culture engineering method which has high application value can be widely used in metabolic engineering field. This paper summarized and discussed the advantages and main challenges of modular co-culture engineering. Meanwhile, this paper summarized the progress of modular co-culture engineering.