| FK506和CSA对异体造血干细胞在粒单白血病小鼠存活及其血清IL-2和IL-4水平的影响 |
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| 引用本文： | 陈静,李春富.FK506和CSA对异体造血干细胞在粒单白血病小鼠存活及其血清IL-2和IL-4水平的影响[J].四川大学学报(医学版),2009,40(3). |
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| 作者姓名： | 陈静 李春富 |
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| 作者单位： | 南方医科大学南方医院,儿科,广州,510515 |
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| 基金项目： | 国家自然科学基金，纽约中华医学基金会资助项目 |
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| 摘 要： | 目的 探讨环孢霉素A (CSA)和FK506对造血干细胞异体移植及其对IL-2和IL-4表达的影响.方法 用WEHI23细胞从尾静脉注入建立小鼠粒单白血病模型.取绿色荧光蛋白转基因鼠的骨髓,体外扩增纯化培养造血干细胞 (HSC), 移植入粒单白血病小鼠,观察FK506和CSA对植入细胞的存活及其对IL-2和IL-4水平的影响.结果 植入的HSC在正常小鼠和粒单白血病模型小鼠仅存活1周,而在经CSA和FK506处理的粒单白血病鼠,HSC可存活至2周, 且后者的存活数较前者增多.此外,IL-2、IL-4水平在FK506和CSA处理组亦较正常和模型小鼠组下调(P<0.05).结论 FK506能有效抑制宿主对植入HSC的排斥作用,其机制可能与降低IL-2、IL-4水平有关.
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| 关 键 词： | 粒单白血病小鼠模型 造血干细胞 细胞移植 |
Effect of FK506 and CSA on the Cell Survival of Engrafted HSC and the Level of IL-2 and IL-4 in Leukemia Mice |
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| CHEN Jing,LI Chun-fu.Effect of FK506 and CSA on the Cell Survival of Engrafted HSC and the Level of IL-2 and IL-4 in Leukemia Mice[J].Journal of West China University of Medical Sciences,2009,40(3). |
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| Authors: | CHEN Jing LI Chun-fu |
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| Affiliation: | CHEN Jing,LI Chun-fu.Department of Pediatric,Nanfang Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To explore the effect of FK506 and CSA on the survival of HSCs and the level of IL-2 and IL-4.MethodsThe WEHI23 cells were injected into vein from tail of mice to set up the leukemia model.The Hematopoietic stem cells(HSCs)were harvested from GFP mice and cultured in vitro,then injected into the vein of leukemia mice.FK506 and CSA,as two immunosuppress agents,were applied in leukemia mice and intact mice,and leukemia mice were as control.Results Some HSCs could survive in the host treated with FK5... |
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| Keywords: | IL-2 IL-4 |
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