光叶子花茎段愈伤组织的诱导及其植株再生的研究

 研究了光叶子花(Bougainvillea glabra Choisy) 茎段愈伤组织的诱导及植株再生。结果表明:愈伤组织诱导以MS +BA 3.0 mg·L ^-1 + 2,4-D 0.2 mg·L^-1 +NAA 0.1 mg·L^-1培养基最好, 茎段愈伤组织的诱导率和分化率分别达78.2%和25.6%。不定芽的产生有两种类型: 直接从茎段基部诱导产生不定芽和茎段基部形成大块愈伤组织后再从愈伤组织上分化出不定芽。丛生苗诱导以MS +BA 2.0 mg·L^-1 +NAA 0.1 mg·L^-1为培养基, 增殖倍数可达5.4, 当培养基中BA 3.0 mg·L^-1和2,4-D 0.5～1.0 mg·L^-1时再生植株会出现叶片变异现象。MS+ BA 0.2 mg·L^-1 + GA₃ 0.5 mg·L^-1 +NAA 0.1 mg·L^-1培养基对有效苗培养具有较好的效果; 生根诱导以MS +NAA 3.0 mg·L^-1培养基诱导率最高, 为88.3%。

Callus Induction and Plant Regeneration from Stem Segment of Bougainvillea glabra Choisy

This papermainly dealt with the studies on callus induction and plant regeneration from stem segment of Bougainvillea glabra Choisy. The results showed that MS medium supplemented with BA 3.0 mg·L^-1 + 2,4-D 0.2 mg· L ^{- 1} +NAA 0.1 mg·L ^{- 1} was best for callus induction in which the induction rate and differentiation rate of callus were 78.2% and 25.6% , respectively. Two types of shoots were observed: the first type of shootswas directly induced from the bottom of stem segment, the second type of shoots differentiated from the callus induced at the bottom of stem segment. MS medium containing BA 2.0 mg·L^-1 +NAA 0.1 mg·L^-1 was best for clump shoots induction in which the ratio of multiplication was 5.4, while BA and 2,4-D increased to 3.0 mg·L^-1 and 0.5 - 1.0 mg·L ^{- 1} respectively would result in leaf variation of regenerated plants. MS medium containing BA 0.2 mg·L ^{- 1} + GA₃ 0.5 mg·L^-1 +NAA 0.1 mg·L ^{- 1} gave best result for efficient seedling culture. MS medium containing NAA 3.0 mg·L ^{- 1} was best for root induction where the rate of rooting was 88.3%.