| 荧光光度法对水解胶原蛋白与铬络合物作用的模拟研究 |
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| 引用本文： | 王应红,陈武勇,辜海彬,陈继平. 荧光光度法对水解胶原蛋白与铬络合物作用的模拟研究[J]. 皮革科学与工程, 2006, 16(5): 14-16 |
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| 作者姓名： | 王应红 陈武勇 辜海彬 陈继平 |
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| 作者单位： | 皮革化学与工程教育部重点实验室(四川大学),四川,成都,610065;皮革化学与工程教育部重点实验室(四川大学),四川,成都,610065;皮革化学与工程教育部重点实验室(四川大学),四川,成都,610065;皮革化学与工程教育部重点实验室(四川大学),四川,成都,610065 |
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| 摘 要： | 通过荧光光度法,用硫酸铬、甲酸钠及甲酸与水解胶原作用,为认识铬鞣及铬鞣机理提供更多的证据。结果表明:水解胶原蛋白中加入硫酸铬溶液,其荧光强度在315 nm处有不同程度的降低;加入硫酸铬溶液的同时,引入相同量的甲酸钠,此时荧光猝灭更明显;在一定条件下,水解胶原的pH对水解胶原荧光猝灭最明显,这可能是pH的变化引起水解胶原疏水性变化的结果。
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| 关 键 词： | 荧光猝灭 水解胶原 铬鞣机理 铬配合物 |
| 文章编号： | 1004-7964（2006）05-0014-03 |
| 收稿时间： | 2006-04-30 |
| 修稿时间： | 2006-04-30 |
Study of Simulation of Reaction of Hydrolysis Collagen and Chromium Complex Compound by Fluorescent Method |
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| WANG Ying-hong,CHEN Wu-yong,GU Hai-bin,CHEN Ji-ping. Study of Simulation of Reaction of Hydrolysis Collagen and Chromium Complex Compound by Fluorescent Method[J]. Leather Science and Engineering, 2006, 16(5): 14-16 |
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| Authors: | WANG Ying-hong CHEN Wu-yong GU Hai-bin CHEN Ji-ping |
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| Affiliation: | Key Laboratory of Leather Chemistry and Engineering (Sichuan University |
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| Abstract: | Chromic sulfate,sodium formate and formate acid react with hydrolysis collagen were investigated by fluorescent method to get the more evidence of understanding chrome tanned mechanics.Results show fluorescenceintensity at 315nm has definite quenching when hydrolysis collagen reacts with chromic sulfate;There have more apparently quenching when hydrolysis collagen reacts with the mixture of chromic sulfate and sodium formate;The change of pH can cause most apparently quenching of hydrolysis collagen,it may be variation of pH result in the alteration of hydrophobicity of hydrolysis collagen. |
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| Keywords: | fluorescence quenching hydrolysis collagen chrome tanned mechanics chromium complex compound |
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