HMGB-1基因及其受体RAGE在宫颈鳞癌中的表达及临床意义

目的:检测高迁移率族蛋白-1(HMGB-1) mRNA和晚期糖基化终产物受体(RAGE) mRNA在宫颈鳞癌(CSEC)组织中的表达及其与临床病理参数间的关系,探讨它们与CSEC发生、侵袭和转移的关系。方法:采用实时荧光定量PCR(qRT-PCR)检测CSEC、癌旁及正常宫颈组织中HMGB-1 mRNA和RAGE mRNA的表达情况。结果:(1)HMGB-1 mRNA在CSES、癌旁组织表达水平均高于正常宫颈组织(P<0.05)。其表达与肿瘤分期、淋巴转移明显相关(P<0.05),而与肿瘤直径、分级无关(P>0.05)。(2)RAGE mRNA在CSES、癌旁及正常的宫颈组织中的表达水平无明显的统计差异(P>0.05),其表达与肿瘤直径、分级、分期无关(P>0.05),仅与淋巴转移相关(P<0.05)。结论:HMGB-1可作为判断宫颈肿瘤侵袭、转移的生物指标,RAGE与CSEC转移相关,HMGB-1/RAGE可能是促进CSEC转移发生的重要信号通路,它们将有可能成为治疗宫颈癌淋巴转移的新靶点。

Gene expression and clinical significance of HMGB-1 and RAGE in cervical squamous epithelial carcinoma

Objective:To investigate the expression and clinico-pathological findings of high mobility group box-1(HMGB-1)mRNA and receptor for advanced glycation end products(RAGE)mRNA in cervical squamous epithelial carcinoma,to explore their roles during the carcinoma process,invasion and metastasis.Methods:Real time quantitative polymerase chain reaction(qRT-PCR)were employed to detect the expression of HMGB-1 mRNA and RAGE in CSEC and nomal cervical tissues.Results:The expression of HMGB-1 mRNA in the CSEC and their paraneoplastic tissues was higher than that in normal cervix tissues.And the overexpression of HMGB-1 mRNA in the CESC tissues was significantly correlated with the stage,the presence of metastasis,but not correlated with the diameter and grade of tumor.And expression of RAGE was no significantly differences between the CSEC,paraneoplastic and normal cervical tissues.Statistical analysis showed that RAGE mRNA had a relation with the metastasis.But no relation in stage,diameter and grade of CSEC.Conclusion:HMGB-1 could be a prognostic predictor of malignant degree in the evaluation of CSEC patients.The pathway of HMGB-1/RAGE is the main mechanism correlated with the metastasis of CESC and they may become new targets for treating CSEC lyphatic metastasis.