| Identification of Nodulation Genes (nod Locus 5) From Azorhizobium caulinoaans ORS571 |
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| 引用本文： | 高盟生,尤崇杓,K.Goethals,M.Holsters. Identification of Nodulation Genes (nod Locus 5) From Azorhizobium caulinoaans ORS571[J]. 中国科学B辑(英文版), 1993, 0(10) |
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| 作者姓名： | 高盟生 尤崇杓 K.Goethals M.Holsters |
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| 作者单位： | Department of Biotechnology，IAAE，Chinese Academy of Agricultural Sciences，Beijing 100094，PRC，Department of Biotechnology，IAAE，Chinese Academy of Agricultural Sciences，Beijing 100094，PRC，Laboratrium vor Genetica，Universiteit Gent.，B-9000 Gent，Belgium，Laboratrium vor Genetica，Universiteit Gent.，B-9000 Gent，Belgium |
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| 摘 要： | An 800 bp AccI-PUCII DNA containing promoter region of nodABC (P_i)from A. caulinodans ORS571 was cloned and used as a hybridization probe against the chrom(?)some DNA, which led to the identification of another 8.4-kb EcoRI fragment showing homology to P_1. A corresponding clone of 8.4-kb DNA was isolated from a pLAFI gene bank (pRG90). The restriction enzyme analysis and DNA-DNA hybridization of 8.4-kbDNA indicated the P_1 homology was located in the 450-bp SmaI-SphI region (P_2 region). Ω insertion deletion of 8.4 kb DNA resulted in a mutant strain ORS571-5 that delayed to nodulate the stems of S. rostrata. This phenotype was complemented by the introduction of pRK84 carrying nod locus 5 DNA.
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| 关 键 词： | nod gene promoter DNA-DNA hybridization Ω factor. |
Identification of Nodulation Genes (nod Locus 5) From Azorhizobium caulinoaans ORS571 |
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| GAO Meng-Sheng YOU Chong-Biao. Identification of Nodulation Genes (nod Locus 5) From Azorhizobium caulinoaans ORS571[J]. Science in China(Chemistry), 1993, 0(10) |
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| Authors: | GAO Meng-Sheng YOU Chong-Biao |
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