一株产乙醇细菌的分离筛选与鉴定

以湖南省盛产的4种刺葡萄为原料,经过分离筛选与鉴定,从米刺葡萄表面获得编号为MUY3的一株产乙醇的细菌。MUY3在28℃发酵48h后,其发酵液中乙醇体积分数可达到10.67%。通过绘制生长曲线、同化试验、甲基红试验、耐高渗透压试验等对其生理生化特征进行研究。同时采用16SrDNA序列鉴定及进化树分析对该细菌进行鉴定。结果表明,MUY3与Enterobacter xiangfangensis strain 10-17最为接近,相似度达100%,所以该菌种为Enterobacter xiangfangensis strain 10-17。

Isolation, screening and identification of an ethanol-producing bacterium

From four kinds of Vitis davidii, widely planted in Hunan Province, an ethanol-producing bacterium named MUY3 was obtained from the surface of the rice grape by separation screening and identification. Its physiological and biochemical characteristics were investigated, including the cultivation of growth curve, assimilation test, methyl red test and high osmotic pressure test. Moreover, its 16S rDNA sequence was analyzed, and the phylogenetic tree analysis were used to identify this bacterium. The results showed that MUY3 was close to Enterobacter xiangfangensis and the similarity was 100%. Therefore, the strain was identified as Enterobacter xiangfangensis 10-17.