| 采用控制酶解法从罗非鱼肉中制备血管紧张素转化酶抑制肽 |
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| 引用本文： | 朱志伟,曾庆孝,林奕封,张立彦,阮征. 采用控制酶解法从罗非鱼肉中制备血管紧张素转化酶抑制肽[J]. 食品与发酵工业, 2004, 30(12): 71-76 |
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| 作者姓名： | 朱志伟 曾庆孝 林奕封 张立彦 阮征 |
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| 作者单位： | 华南理工大学食品与生物工程学院,广州,5106400 |
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| 基金项目： | 广东省科技厅项目资助(课题编号2002C20314)，广东省海洋渔业局项目资助(课题编号A2200099H01) |
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| 摘 要： | 实验以罗非鱼肉为材料,利用Trypsin、Alcalase2.4L、Protamex、木瓜蛋白酶、中性蛋白酶、Flavourzyme和Kojizyme酶解制备血管紧张素转化酶抑制肽(ACEIP)。以ACE抑制率(Ⅰ值)、氨基酸态氮含量(Cn值)和TCA可溶性蛋白(短肽)含量(Cp值)为指标对酶解过程进行分析,说明了酶法水解以及不同酶水解生成产物的特点,指出控制酶解的方法,其中Trypsin、Alcalase2.4L、Protamex对罗非肉控制酶解制备ACEIP的效果较好,酶解产物(稀释20倍)的最大Ⅰ值分别为酶解1hⅠ值91.2%、酶解3hⅠ值90.9%,酶解产物对ACE的抑制率比较高。
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| 关 键 词： | 罗非鱼 酶解 血管紧张素转化酶 抑制率 |
| 修稿时间： | 2004-04-27 |
Study on ACEIP Preparation by Controlled Hydrolysis of Tilaplia Meat |
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| Zhu Zhiwei,Zeng Qingxiao,Lin Yifeng,Zhang Liyan,Ruanzheng. Study on ACEIP Preparation by Controlled Hydrolysis of Tilaplia Meat[J]. Food and Fermentation Industries, 2004, 30(12): 71-76 |
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| Authors: | Zhu Zhiwei Zeng Qingxiao Lin Yifeng Zhang Liyan Ruanzheng |
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| Abstract: | Hydrolysis by Trypsin,Alcalase2.4L,Protamex,papain proteinase,neutral proteinas,Flavourzyme and Kojizyme for tilaplia meat were studied. The content of amino nitrogen(C_n),the content of TCA soluble peptide(C_p) and the ACE inhibitor rate(Ⅰ) were analyzed during the hydrolyzing. The author reviewed the properties of hydrolysis by proteinase and product during the hydrolyzing and presented the controlled hydrolysis technology. After 1h,1h and 3h by hydrolysis of Trypsin、Alcalase2.4L、Protamex, the hydrolyzed samples with ACE inhibitor rate 91.2% and 90.9%(when diluted 20 times) were obtained respectively. |
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| Keywords: | tilaplia hydrolysis Angiotensin Converting Enzyme(ACE) inhibitory rate |
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