| 唾液链球茵尿素酶结构亚基的克隆和表达 |
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| 引用本文： | 栾晓玲,王艳,冯希平. 唾液链球茵尿素酶结构亚基的克隆和表达[J]. 口腔医学研究, 2011, 27(7): 549-551,554 |
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| 作者姓名： | 栾晓玲 王艳 冯希平 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院口腔预防儿童科·上海市口腔重点实验室,上海,200011 |
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| 基金项目： | 国家自然科学基金资助(编号:30572038); 上海市自然科学基金资助(编号:08ZR1416800); 上海市重点学科建设项目资助(编号:S30206); 上海市科学技术委员会资助项目(09DZ2272100) |
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| 摘 要： | 目的：克隆和表达唾液链球菌尿素酶结构亚基UreA、UreB、UreC。方法：用已构建的含唾液链球菌57．I尿素酶基因簇的重组质粒为模板，分别克隆、双酶切、连接、转化，构建含结构基因的表达质粒，IPTG诱导表达，亲和层析纯化蛋白。结果：成功构建表达质粒，经诱导、亲和层析，获得高纯度、高浓度的UreA、UreB、UreC3种目的蛋白。结论：成功诱导纯化唾液链球菌尿素酶的结构亚基，为今后各亚基的酶活分析和抗体制备等研究奠定基础。
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| 关 键 词： | 克隆 表达 唾液链球菌 尿素酶 结构亚基 |
Cloning and Expression of Structural Subunits of Streptococcus Salivarius Urease |
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| LUAN Xiao-ling,WANG Yan,FENG Xi-ping. Cloning and Expression of Structural Subunits of Streptococcus Salivarius Urease[J]. Journal of Oral Science Research, 2011, 27(7): 549-551,554 |
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| Authors: | LUAN Xiao-ling WANG Yan FENG Xi-ping |
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| Affiliation: | LUAN Xiao-ling,WANG Yan,FENG Xi-ping.Department of Preventive & Pediatric Dentistry,The Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology,Shanghai 200011 |
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| Abstract: | Objective:To clone and express the structural subunits of Streptococcus salivarius urease of UreA,UreB,UreC.Methods:The expression plasmids were constructed by using the recombinant plasmid with the urease gene cluster of Streptococcus salivarius 57.I as the template.The expression was induced by IPTG,and the purification was through affinity chromatography.Results:The expression plasmids were successfully constructed.The proteins were highly expressed by the induction of IPTG and significantly purified thr... |
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| Keywords: | Cloning Gene expression Streptococcus Urease Structural subunits |
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