| 铁皮石斛茎尖脱毒组培快繁技术研究 |
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| 引用本文： | 何晔,陈明,王运娥.铁皮石斛茎尖脱毒组培快繁技术研究[J].安徽农业科学,2013(24):9917-9917,9984. |
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| 作者姓名： | 何晔 陈明 王运娥 |
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| 作者单位： | 济宁森立生物科技有限公司,山东微山277600;微山县农业局,山东微山277600;微山县农业局,山东微山,277600;微山县第一中学,山东微山,277600 |
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| 摘 要： | 目的]研究铁皮石斛的茎尖脱毒组培快繁技术.方法]参考其他作物的脱毒技术对铁皮石斛进行脱毒组培研究.结果]培养基为MS＋ 0.2 mg/L NAA＋ 0.3 mg/L 6-BA时,茎尖的愈伤组织诱导率和分化率最高.结论]该方法为今后进一步探讨铁皮石斛的脱毒组培快繁技术奠定了基础.
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| 关 键 词： | 铁皮石斛 茎尖脱毒 组织培养 |
Study on the Detoxification and Rapid Propagation of Stem Tip from Dendrobium candidum Wall.ex Lindl. |
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| Affiliation: | HE Ye et al (Jining Senli Biological Technology Co. , Ltd. , Weishan, Shangdong 277600) |
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| Abstract: | Objective] The aim was to study the detoxification and rapid propagation technology of stem tip of Dendrobium candidum Wall. ex Lindl.. Method] Reference to the detoxification technology of other crops, the detoxification and tissue culture of D. candidum was studied. Result] The callus induction rate and differentiation rate of stem tip were highest when the medium was MS ＋ 0.2 mg/L NAA ＋ 0.3 mg/L 6-BA. Conclusion The method laid a foundation for the further study of detoxification and rapid propagation technology of D. candidum. |
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| Keywords: | Dendrobitum candidum Wall ex Lindl Detoxification of stem tip Tissue culture |
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