| 固相萃取-HPLC—PDA法测定猪肉中的盐酸克伦特罗的研究 |
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| 引用本文： | 韩巍,张文治. 固相萃取-HPLC—PDA法测定猪肉中的盐酸克伦特罗的研究[J]. 齐齐哈尔轻工业学院学报, 2012, 0(3): 28-30,39 |
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| 作者姓名： | 韩巍 张文治 |
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| 作者单位： | [1]黑龙江八一农垦大学测试中心,黑龙江大庆163319 [2]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006 |
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| 摘 要： | 对固相萃取——HPLC—PDA法测定猪肉中的盐酸克伦特罗的前处理方法和色谱条件进行了优化。超声提取时间30min，固相萃取柱净化过柱速度为2d／s。色谱条件为V甲醇：V0.01mool/L磷酸二氯钠溶液=35：65，固定相ODS—C18，高效液相色谱柱4．6μm×150mm，流速1mL/min，检测波长243nm。优化试验条件后盐酸克伦特罗残留量含量回收率得到较大改善．该方法检测线性良好，操作简单、快速，色谱峰形理想，结果准确可靠，重现性佳。
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| 关 键 词： | 固相萃取 HPLC—PDA法 盐酸克伦特罗 |
Optimization of detection of clenbuterol residue in pork by solid phase Extraction-HPLC method |
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| HAN Wei,ZHANG Wen-zhi. Optimization of detection of clenbuterol residue in pork by solid phase Extraction-HPLC method[J]. , 2012, 0(3): 28-30,39 |
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| Authors: | HAN Wei ZHANG Wen-zhi |
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| Affiliation: | 1. Test Center, Heilongjiang Bayi Agricultural University, Heilongjiang Daqing 163319, China; 2. Chemistry and Chemieal Engineering College, QiqiharUniversity, HeilongjiangQiqihar 161006, China) |
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| Abstract: | In this paper, solid-phase extraction--RP-HPLC was applied for the detection of clenbuterol residue in pork and both the pre-treatment method and chromatographic conditions were optimized. For the pre-treatment of the meat samples, the best ultrasonic extraction time and elution speed of the extracts on solid phase were 30 minutes and 2d/s, respectively. Then the purified extracts was analyzed by RP-HPLC on a ODS C-18 column （ 4.6 μm × 150 mm）using 0.01mol/L of solium dihydrogen phosphate 1mL/min and a UV detector at 243nm. This method residual recovery, and good reproducibility. solution-methanol（65 ： 35）as mobile phase at a flow rate of for clenbuterol residue detection in pork had high accuracy,residual recovery, and good reproducibility. |
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| Keywords: | solid-phaseextraction HPLC-PDA clenbuterolresidues |
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