磁性附着体静磁场对人牙周膜细胞骨架影响的研究

目的 研究牙科磁性附着体静磁场对人牙周膜细胞骨架的影响.方法 采用细胞静磁场加载装置,模拟磁性附着体静磁场,对体外培养的人牙周膜细胞加载10 mT、120 mT静磁场12～60 h,采用激光扫描共聚焦显微镜观察细胞骨架形态,图像分析软件测定分析细胞面积、长宽比以及微丝蛋白(F-actin)含量.结果 加载10 mT、120 mT静磁场12～60 h后,随加载强度和加载时间增加,细胞微丝骨架变短,排列紊乱,细胞长宽比减小(P＜0.05),加载静磁场60 h后细胞面积减小(P＜0.05),加载12 h后F-actin含量较对照组下降(P＜0.05),而加载36、60 h后与对照组之间差异无统计学意义(P＞0.05).结论 磁性附着体静磁场在本实验条件下能使人牙周膜细胞细胞骨架发生一定程度的改变.

The Research for Magnetic Attachments Producing Static Magnetic Fields to Influence on Cytoskeletons of Human Periodontal Ligament Cells

Objective The present research aimed to investigate that the static magnetic field(SMF)generated by dental magnetic attachments influenced on the cytoskeletons of human periodontal ligament cells(HPDLCs).Methods In vitro cultured HPDLCs were exposed to 10 mT and 120 mT SMF which simulated those of from the closed-field and open-field magnetic attachments respectively for 12-60 hours in a cellular SMF exposure system.The control group cells were cultured outside the SMF exposure system and were only exposed to geomagnetic field of about 0.03-0.07 mT.After finishing the SMF exposure,the cytoskeletons were observed under a laser scanning confocal microscope(LSCM).The image analysis software was utilized to measure and analyze the cell area,length/width ratios and the contents of F-actin of cells.Results There were certain changes observed in the cytoskeletons after HPDLCs exposed to 10 mT and 120 mT SMF for 12-60 hours.With the SMF strength increasing and the loading time extending,the cell microfilaments got ashorened change and a disordered arrangement inside cells,and the length/width ratios of cells decreased(P<0.05).The areas of cells decreased after SMF loading for 60 hours(P<0.05).The cell F-actin contents decreased after SMF loading for 12 hours(P<0.05),but in the meantime there was no significant difference happening between the 36 or 60 hours SMF loading group and the control group(P>0.05).Conclusion In this study the SMF of magnetic attachments can lead the certain changes to the cytoskeletons in HPDLCs.