| 黄芪甲苷对OVA诱导哮喘小鼠Th细胞亚群的影响 |
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| 引用本文： | 王嵩,郤庆,李国文,昌军,俞培忠,沈朝斌.黄芪甲苷对OVA诱导哮喘小鼠Th细胞亚群的影响[J].上海中医药杂志,2017,51(12):67-70. |
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| 作者姓名： | 王嵩 郤庆 李国文 昌军 俞培忠 沈朝斌 |
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| 作者单位： | 上海中医药大学附属上海市中西医结合医院,上海,200082;复旦大学,上海,201203,上海中医药大学附属上海市中西医结合医院,上海,200082,上海中医药大学附属上海市中西医结合医院,上海,200082,复旦大学,上海,201203,上海市中医老年医学研究所,上海,200031,上海中医药大学附属上海市中西医结合医院,上海,200082 |
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| 基金项目： | 上海市卫计委进一步加快中医药事业发展三年行动计划项目(2014年-2016年)(ZY3-RCPY-3-1038)；上海中医药大学预算内项目(2014YSN78) |
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| 摘 要： | 目的观察黄芪甲苷(AST IV)对卵白蛋白(OVA)诱导的哮喘小鼠脾源性Th1及Th2的免疫调节作用。方法建立OVA诱导的小鼠哮喘模型,分离培养脾单个核细胞,加入不同浓度的黄芪甲苷。采用酶联免疫吸附法(ELISA)测定细胞培养液上清液细胞因子IFN-γ、IL-4及IL-13的表达;流式细胞术(FCM)在单个核细胞内测定IFN-γ、IL-4及IL-13的百分含量。结果与OVA组比较,不同剂量黄芪甲苷(25 mg/L、50 mg/L、100 mg/L)对OVA诱导的哮喘小鼠Th细胞亚群分泌的细胞因子IFN-γ、IL-4及IL-13均有一定的抑制作用(P0.05)。结论黄芪甲苷对OVA诱导的Th2细胞应答有抑制作用,通过抑制Th1及Th2细胞达到免疫调节作用。
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| 关 键 词： | 黄芪甲苷 免疫调节 体外实验 Th细胞亚群 |
| 收稿时间： | 2017/1/23 0:00:00 |
Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice |
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| WANG Song,XI Qing,LI Guowen,CHANG Jun,YU Peizhong and SHEN Chaobin.Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice[J].Shanghai Journal of Traditional Chinese Medicine,2017,51(12):67-70. |
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| Authors: | WANG Song XI Qing LI Guowen CHANG Jun YU Peizhong and SHEN Chaobin |
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| Affiliation: | Shanghai Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China;Fudan University,Shanghai 201203,China,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China,Shanghai Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China,Fudan University,Shanghai 201203,China,Shanghai Geriatric Institute of Chinese Medicine,Shanghai 200031,China and Shanghai Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China |
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