| 琼脂糖微滴法测定白细胞化学运动 |
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| 引用本文： | 张克坚. 琼脂糖微滴法测定白细胞化学运动[J]. 中国医科大学学报, 1985, 0(4) |
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| 作者姓名： | 张克坚 |
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| 作者单位： | 中国医科大学病理生理学教研室 |
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| 摘 要： | 本文介绍了一种新的体外测定白细胞化学运动的方法。这种琼脂糖微滴法具有操作简单,需血量少,结果稳定等优点。本法可用于免疫学、病理生理学及药理学等学科的研究工作。本实验选用人工合成肽fMLP做为标准的化学激动因子,其对大鼠外周血白细胞引起最强化学运动的浓度为5nM。孵育时间以4小时为宜。
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| 关 键 词： | 化学运动 白细胞 |
AGAROSE MICRODROFLET ASSAY FOR CHEMOKINESIS OF LEUCOCYTE |
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| Zhang Kejian,et al. AGAROSE MICRODROFLET ASSAY FOR CHEMOKINESIS OF LEUCOCYTE[J]. Journal of China Medical University, 1985, 0(4) |
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| Authors: | Zhang Kejian et al |
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| Affiliation: | Zhang Kejian,et al Department of pathophysiology |
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| Abstract: | A new in vitro assay for measuring chemokinesis of leucocyte is described.This agarose microdroplet assay is simple, reproducible, and requires onlysmall amount of blocd. This method may be applied in immunological, pa-thophysiological and pharmacolcgical investigations. In this test, the syntheticpeptid fMLP (formylmethionyl-leucyl-pheny lalanine) was used as standardchemokinetic factor. Maximal chemokinetic movement was observed with fMLPconcentration of 5nM for rat peripherial blocd leucccyte. Time of incubationwas controlled in 4 hours. |
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| Keywords: | Chemokinesis Leucocyte |
| 本文献已被 CNKI 等数据库收录！ |
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