PCR-mediated site-directed mutagenesis on PedB gene and HaeIII restriction as a rapid tool for discrimination among pediocin AcH/PA-1 producer strains |
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| Affiliation: | 1. Department of Obstetrics and Gynecology, Putrajaya Hospital, Wilayah Persekutuan Putrajaya, Malaysia;1. Radiation Metrology Center, Instituto de Pesquisas Energéticas e Nucleares – IPEN/CNEN, Av. Prof. Lineu Prestes, 2242 – Cidade Universitária, São Paulo, SP, Brazil;2. Radiation Therapy Department, Hospital Israelita Albert Einstein, Av. Albert Einstein, 665 – Morumbi, São Paulo, SP, Brazil |
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| Abstract: | Nucleotide sequences of amplified pedB genes from Pediococcus parvulus andLactobacillus plantarum pediocin AcH/PA-1 producer strains were performed. The obtained data were aligned with the published Pediococcus acidilactici pedB sequence, showing a single base substitution in the third codon position of a putative serine. A PCR-mediated site directed mutagenesis on pedB gene, followed by Hae III restriction analysis was then carried out with the aim to rapidly discriminate among pediocin AcH/PA-1 producer strains of different species. |
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