Megaplasmid encoding novel sugar utilizing phenotypes,pediocin production and immunity in Pediococcus acidilactici C20 |
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| Affiliation: | 1. Laboratório de Radiobiologia Molecular, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, Brazil;2. Laboratório de Microbiologia e Avaliação Genotóxica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, Brazil;3. Laboratório de Tecnologia Industrial Farmacêutica, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, Brazil;4. Laboratório de Modelagem Molecular and QSAR-3D, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, Brazil;5. Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil |
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| Abstract: | A bacteriocinogenic strain of Pediococcus acidilactici C20 isolated from fowl intestine was found to bear a single megaplasmid. Strain C20 exhibited an ability to ferment a wide range of sugars including lactose, maltose and melibiose. Curing experiments employing novobiocin and high temperature indicated that the genetic determinants for pediocin production, immunity function and utilization of several sugars resided on the megaplasmid. Pediocin production with different sugars was tested and culture filtrate activity varied from 2500 AU ml−1to 3500 AU ml−1at 1% of carbon source. Molecular and biochemical techniques were used to characterize the bacteriocin produced by strain C20. It was found to be a pediocin AcH/PA-1 like bacteriocin with high antilisterial activity. |
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