| 长链烷基化物与牛血清白蛋白相互作用的共振光散射研究 |
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| 引用本文： | 奉萍,黄承志,舒为群.长链烷基化物与牛血清白蛋白相互作用的共振光散射研究[J].环境化学,2001,20(5):497-502. |
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| 作者姓名： | 奉萍 黄承志 舒为群 |
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| 作者单位： | 1. 西南师范大学环境化学研究所重庆市三峡库区水系
2. 第三军医大学预防医学系, |
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| 基金项目： | 国家自然科学基金(批准号:2987519)和重庆科技攻关项目(批准号99－5772)资助 |
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| 摘 要： | 应用荧光分光度计进行共振光散射测定，研究了水相介质中长链烷基化物十二烷基硫酸钠（SLS）与牛血清白蛋白（BSA）的结合平衡。实验表明，在微酸性条件下，SLS与BSA形成的复合物引起共振散射增强，根据470mm的光散射增强信号建立了一定酸度下SLS与BSA结合的数学关系式，随着pH值的变化，二者相互作用的结合平衡常数（Kf）及每分子BSA所结合的SLS个数（n）均不相同。
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| 关 键 词： | 十二烷基硫酸钠 牛血清白蛋白 共振光散射 合成洗涤剂 废水处理 废水监测 |
A STUDY ON THE INTERACTION OF LONG CHAIN ALKYL COMPLEX WITH BOVINE SERUM ALBUMIN AS MEASURED BY RESONANCE LIGNHT SCATTERING |
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| Feng Ping,Huang Chengzhi.A STUDY ON THE INTERACTION OF LONG CHAIN ALKYL COMPLEX WITH BOVINE SERUM ALBUMIN AS MEASURED BY RESONANCE LIGNHT SCATTERING[J].Environmental Chemistry,2001,20(5):497-502. |
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| Authors: | Feng Ping Huang Chengzhi |
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| Abstract: | The binding of sodium laury sulfate(SLS) to bovine serum albumin(BSA) was studied byresonance light scattering in the present paper. It was showed that the interaction results in stronglyenhanced resonance light scattering signals characterized by the are peak at 470 nm.The bindingconstant Kf and the number of binding n were calculated according to the equilibrium equation,which was established based on the data of the resonance light-scattering measurements at 470 nm.It was found that both binding constatn Kf and the number of binding n were changed with varyingpH. |
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| Keywords: | sodium lauryl sulfate (SLS) bovine serum albumin (BSA) resonance light-scattering (RLS) |
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