日本猴椎旁交感神经节中含肽神经元的研究

本实验系应用荧光免疫组织化学的方法观察猴下位腰段椎旁交感神经节(L_(6-7))中神经肽Y,血管活性肠肽,降钙素基因相关肽,和P物质的存在、分布情况以及它们与酪氨酸羟化酶的共存关系。结果表明,大量细胞呈神经肽Y免疫反应阳性,它们在神经节周边分布更为密集。中等数量的血管活性肠肽阳性细胞和小量降钙素基因相关肽细胞散在于神经节内。在经含有Colchiciue的培养液离体孵育12h的标本上,可见中等数量的P物质免疫反应阳性细胞。根据抗酪氨酸羟化酶(TH)抗体的免疫染色结果,神经节内的神经元可分为TH~+和TH~-两群,前者占大多数。相邻切片免疫染色结果表明,几乎所有神经肽Y免疫阳性细胞同时含有TH,而所有血管活性肠肽免疫反应阳性细胞均呈酪氨酸羟化酶免疫反应阴性。神经肽Y与血管活性肠肽无共存关系。降钙素基因相关肽存在于部分血管活性肠肽免疫反应阳性细胞中,即属于VIP~+/TH~-组。从以上结果得出结论,在猴下位腰段椎旁交感神经节中,神经肽Y与血管活性肠肽分别存在于TH~+和TH~-两个细胞群。即神经肽Y存在于TH阳性神经元中,血管活性肠肽和降钙素基因相关肽则存在于TH阴性神经元中。

A STUDY OF PEPTIDE-CONTAINING NEURONS IN THE SYMPATHETIC GANGLIA OF JAPANESE MONKEY

The distributions of NPY, VIP, CGRP immunoreactivity and coexistance of these peptides with Tyrosine Hydroxylase(TH) were investigated in the lower lumbar paravertebral sympathetic ganglia (L6, L7) of Japanese monkey by using indirect immunofluoreseence technique. A great number of NPYlike immunoreactive (LI) neurons were discovered in the ganglia. They were mainly distributed in the peripheral area of the ganglia, while a moderate number of VIP-LI neurons and a small number of CGRP-LI neurons were scattered within these ganglia. In the ganglia pre-ineubated with colchicine-containing medium for 12 hours a number of weakly stained SP-LI neurons were demonstrated. Based on their immunoreactivity to TH, the neuron s in the sympathetic ganglia could be classified into two groups: TH-positive and TH-negative. The majority of neurons in our investigation were TH-positive. It was revealed by consecutive section obser ration that almost all the NPY-LI neurons showed positve immunoreactivity of TH, whereas all of VIP-LI neurons were negative. No coexistence of NPY and VIP was found. CGRP-LI neurons were proved to be VIP-immttnoreactive. This supported that CGRP-LI neurons belonged to the TH negative group. The results suggested that in the lower lumbar paravertebral sympathetic ganglia, NPY-LI neurons and VIP-LI plus CGRP-LI neurons belonged to the TH positive and TH negative groups respectively. That is to say, NPY was contained in the adrenergic neurons, while VIP and CGRP were assumed to be in non-adrenergic neurons.