| 理中汤、人参皂苷Rg1、6-姜酚对Caco-2细胞PepT1转运功能影响的比较 |
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| 引用本文： | 郭文峰,胡灿,刘佳,高小玲,陈蔚文.理中汤、人参皂苷Rg1、6-姜酚对Caco-2细胞PepT1转运功能影响的比较[J].亚太传统医药,2011,7(8):7-10. |
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| 作者姓名： | 郭文峰 胡灿 刘佳 高小玲 陈蔚文 |
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| 作者单位： | 广州中医药大学脾胃研究所,广东广州,510405 |
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| 摘 要： | 目的:观察比较理中汤、人参皂苷Rg1、6-姜酚对Caco-2细胞肽转运载体PepT1转运二肽的能力及其蛋白、mRNA表达的影响。方法:Caco-2细胞融合后连续培养28d后分别给予理中汤、人参皂苷Rg1、6-姜酚处理,并设立正常对照组及cAMP抑制剂对照组,用放射性同位素示踪技术比较Caco-2细胞转运二肽化合物Glycyl-Sarcosine的能力,采用westernblot方法测定Caco-2细胞膜上PepT1蛋白的表达,荧光定量PCR方法测定PepT1mRNA(SLC15A1)的表达水平。结果:理中汤、人参皂苷Rg1及6-姜酚均在不同程度上表现为促进Caco-2细胞转运Glycyl-Sarcosine的作用;Caco-2细胞经理中汤/人参皂苷Rg1/6-姜酚处理24h后,细胞膜PepT1蛋白表达水平均明显增高。6-姜酚能明显上调Caco-2细胞SLC15A1表达,人参皂苷对Caco-2细胞SLC15A1表达的作用表现为下调,理中汤对Caco-2细胞SLC15A1表达作用不明显。且理中汤、人参皂苷Rg1、6-姜酚三者作用各有特点。结论:理中汤及其成分人参皂苷Rg1、6-姜酚具有促进正常培养Caco-2细胞转运二肽化合物Glycyl-Sarcosine的作用,该过程调控与胞内第二信使cAMP有一定的关系。
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| 关 键 词： | 理中汤 人参皂苷Rg1 6-姜酚 肽转运载体1 环磷酸腺苷 |
Comparison of Lizhong Decoction, Ginsenoside Rg1 and 6-Gingerol on the Transport Ability of PepT1 in Caco-2 Cells |
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| Guo Wenfeng,Hu Can,Liu Jia,GaoXiaoling,Cheng Weiwen.Comparison of Lizhong Decoction, Ginsenoside Rg1 and 6-Gingerol on the Transport Ability of PepT1 in Caco-2 Cells[J].Asia-Pacific Traditional Medicine,2011,7(8):7-10. |
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| Authors: | Guo Wenfeng Hu Can Liu Jia GaoXiaoling Cheng Weiwen |
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| Affiliation: | Guo Wenfeng,Hu Can,Liu Jia,GaoXiaoling,Cheng Weiwen(1.Piwei Institute,Guangzhou University of Chinese Medicine,Guangdong 510405,China) |
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| Abstract: | Objective:The aims of this study were to observe the effects of 6-gingerol on expression and transport ability of PepT1 in Caco-2 cells.Methods:Caco2 monolayers were grown on permeable supports.Peptide transport activity was studied using-glycyl-sarcosine(-Gly-Sar).The densities of PepT1 protein and mRNA(SLC15A1)expression levels were analyzed by Western blot and Real-time quantitative Polymerase Chain Reaction.Results:The total transported Gly-Sar of Caco-2 cells treated by Lizhong Decoction,Ginsenoside Rg... |
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| Keywords: | Lizhong Decoction Ginsenoside Rg1 6-Gingerol Peptide Transporter 1 Cyclic Adenosine Monophosphate |
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