| 咪唑乙烟酸降解菌Y发酵培养基的优化 |
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| 引用本文： | 马超,李晓雨,刘亚光.咪唑乙烟酸降解菌Y发酵培养基的优化[J].东北农业大学学报,2010,41(6). |
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| 作者姓名： | 马超 李晓雨 刘亚光 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 哈尔滨市科技攻关计划项目，大豆生物学省部共建教育部重点实验室开放基金项目 |
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| 摘 要： | 采用摇瓶培养法对咪唑乙烟酸降解菌株Y的发酵培养基配方进行了研究。通过单因子试验确定咪唑乙烟酸降解菌株Y的碳源、氮源及无机盐,并应用正交试验设计对发酵培养基组分进行优化。优化后确定的发酵培养基的配方为:麦麸皮2%,米糠2.5%,豆粕2%,酵母粉1%,NaCl0.8%,CaCl20.6%,FeSO40.015%,文章为大规模发酵生产奠定了基础。
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| 关 键 词： | 咪唑乙烟酸 降解菌 发酵培养基 优化 |
Optimization of imazethapyr degradation bacteria Y fermentation medium |
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| MA Chao,LI Xiaoyu,LIU Yaguang.Optimization of imazethapyr degradation bacteria Y fermentation medium[J].Journal of Northeast Agricultural University,2010,41(6). |
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| Authors: | MA Chao LI Xiaoyu LIU Yaguang |
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| Abstract: | Study on the fermentation medium of imazethapyr degradation bacteria Y by shake-flask experiment. The carbon sources, nitrogen sources and inorganic salt of imazethapyr degradation bacteria Y were determined by single factor experiment. The factors of fermentation medium were optimized by orthogonal design. The optimal fermentation medium composed of 2%wheat bran, 2.5% rice bran, 2% soybean meal, 1% yeast, 0.8% NaCl, 0.6% CaCl2, and 0.015% FeSO4. The medium will lay a foundation for large scale fermentation production of imazethapyr degradation bacteria Y. |
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| Keywords: | imazethapyr degrading microorganism fermentation medium optimization |
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