复合酶法提取红雪茶粗多糖工艺优化研究

为了获得复合酶法提取红雪茶粗多糖的最佳工艺,采用单因素实验和正交实验,研究了不同料液比、pH、酶解温度、提取时间和不同复合酶配比对红雪茶粗多糖提取率的影响;在此基础上采用L9(34)正交实验研究了各影响因素对红雪茶粗多糖提取率的影响,结果表明复合酶最佳配比为纤维素酶2.0%,果胶酶2.0%,木瓜蛋白酶0.5%;影响红雪茶粗多糖提取率的四个因素的主次顺序为:料液比>酶解温度>pH>酶解时间;最佳提取工艺条件是料液比1:40,pH4.5,酶解温度40℃,酶解时间80min,在此条件下红雪茶多糖提取率达8.91%。本研究确定了复合酶法提取红雪茶多糖的最佳工艺。 

Optimization of polysaccharide extraction from Lethariella Cladonioides by compound enzymes method

To obtain the optimized process parameter of extracting the polysaccharide from Lethariella Cladonioides,single factor experiment and orthogonal test were adopted.The effect of different solid-liquid ratio,pH,temperature of enzymatic hydrolysi(cellulase,Pectinase,papain),extracting time and compound enzymes mixture ratio on the extraction rate of polysaccharide from Lethariella Cladonioides were studied.The results showed that compound enzyme best ratio was 2.0% cellulase,2.0% pectinase,0.5% papain.The impact factors were as following:solid-liquid ratio>temperature>pH>time.The optimized process parameter was solid-liquid ratio 1:40,pH4.5,temperature 40℃,time 80min.Under this condition the extraction rate of polysaccharide reached to 8.91%.This method was suitable for polysaccharide extraction from Lethariella Cladonioides.