吡非尼酮对人增生性瘢痕成纤维细胞胶原蛋白分泌的影响

目的 观察吡非尼酮(PFD)对人增生性瘢痕成纤维细胞胶原蛋白分泌的影响.方法 人增生性瘢痕标本取自整形患者,采用组织块法培养人增生性瘢痕成纤维细胞.根据PFD用药的不同浓度分为对照组(0mg·mL-1),实验1组(0.15mg·mL-1)、实验2组(0.3mg·mL-1)及实验3组(1mg·mL-1),加药24、48、72h后,提取细胞上清,EIISA法检测PFD干预下体外培养的人增生性瘢痕成纤维细胞Ⅰ、Ⅲ胶原蛋白的分泌情况.结果 与对照组比较,各实验组对人增生性瘢痕成纤维细胞Ⅰ、Ⅲ型胶原蛋白分泌有明显的抑制作用,呈浓度依赖性,随着药物浓度的增大,抑制作用明显增强(均P＜0.05),但对胶原蛋白分泌抑制作用与干预时间的延长无明显的关系(均P＞0.05).结论 PFD对人增生性瘢痕成纤维细胞胶原蛋白的分泌有明显的抑制作用,对增生性瘢痕的防治有一定意义.

Effect of Pirfenidone on Collagen Secretion from Human Hypertrophic Scar Fibroblasts

Objective To investigate the effect of pirfenidone on collagen secretion from human hypertrophic scar fibroblasts. Methods Human hypertrophic scar fibroblasts obtained from patients who received plastic surgery were cultured in vitro using tissue culture method and were treated with different concentrations of pirfenidone （0.15, 0.3 and 1 mg·mL-1）. Control fibroblasts only received the vehicle. The secretion of type I and Ⅲ collagen from human hypertrophic scar fibroblasts was detected by ELISA 24, 48 and 72 hours after treatment with pirfenidone. Results Compared with control, pirfenidone significantly inhibited the secretion of type I and m collagen in a dose-dependent manner （all P〈0.05）. However, there was no significant relationship between collagen secretion and pirfenidone treatment time（P〈0.05）. Conclusion Pirfenidone can significantly inhibit collagen secretion from hu- man hypertrophic scar fibroblasts. Therefore, it has a certain significance for the prevention and treatment of hypertrophic scars.