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| 对蛴螬有活性的苏云金芽胞杆菌菌株的筛选与基因鉴定 | |
| 引用本文： | 茅洁瑜,束长龙,李克斌,刘春琴,林毅,宋福平,张杰. 对蛴螬有活性的苏云金芽胞杆菌菌株的筛选与基因鉴定[J]. 中国生物防治, 2011, 27(2): 176-181 |
| 作者姓名： | 茅洁瑜 束长龙 李克斌 刘春琴 林毅 宋福平 张杰 |
| 作者单位： | 1. 华侨大学化工学院,厦门,361021;中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 3. 河北省沧州市农林科学院,沧州,061001 4. 华侨大学化工学院,厦门,361021 |
| 基金项目： | 转基因生物新品种培育科技重大专项(2009ZX08009-030B); 公益性行业(农业)科研专项(201003025); 国家“863”计划项目(2011AA10A203) |
| 摘 要： | 为了寻找对地下害虫蛴螬有杀虫活性的基因资源,以实验室分离的500株苏云金芽胞杆菌(Bacillus thuringiensis,Bt)菌株为材料,通过杀虫活性测定筛选到43株对蛴螬有活性的菌株,其中菌株C9对3种供试昆虫均有活性.光学及扫描电镜检测发现这些菌株均释放球形晶体.SDS-PAGE分析显示这些菌株均表达约130kD的蛋白.采用3种方法分析43株菌株的杀虫晶体蛋白基因类型,结果显示:其中21株只含cry8Ca;13株同时含cry8Ca和cry8Ea基因;QC4等8株菌株只能用引物HRM8F/HRM8R获得高度保守区域约110bp的扩增产物,说明这些基因的5'端序列与已知基因差异较大.测序结果经BLAST分析显示,产物序列同cry8基因的相似性均在98%以上,但其全长基因类型还需要其它方法确定.菌株QCM的PCR-RFLF图谱不同于已发现的cry8基因,推测其中含有新基因. |
| 关 键 词： | 蛴螬 苏云金芽胞杆菌 PCR-RFLP cry8基因 |
Screening and Identification of cry Gene from Bacillus thuringiensis Strains Toxic to the Larvae of Cockchafers |
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| MAO Jie-yu , SHU Chang-long , LI Ke-bin , LIU Chun-qin , LIN Yi , SONG Fu-ping , ZHANG Jie. Screening and Identification of cry Gene from Bacillus thuringiensis Strains Toxic to the Larvae of Cockchafers[J]. Chinese Journal of Biological Control, 2011, 27(2): 176-181 | |
| Authors: | MAO Jie-yu SHU Chang-long LI Ke-bin LIU Chun-qin LIN Yi SONG Fu-ping ZHANG Jie |
| Affiliation: | MAO Jie-yu1,2,SHU Chang-long2,LI Ke-bin2,LIU Chun-qin3,LIN Yi1,SONG Fu-ping2,ZHANG Jie2* (1.College of Chemical Engineering,Huaqiao University,Xiamen 361021,2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,CAAS,Beijing 100193,3.Cangzhou Academy of Agricultural and Forestry Sciences,Cangzhou 061001,China) |
| Abstract: | To identify gene resources toxic to cockchafers,500 strains of Bacillus thuringiensis isolated in our laboratory were investigated.Forty-three strains had insecticidal activity against cockchafers,and C9 was entomocidal to all the three species of cockchafers tested.Results of optical and scanning electronic microscope observation indicated that all of these Bt strains contained crystals in spherical shape.SDS-PAGE analysis indicated that all these strains could express protein with 130 kD molecular mass.Th... |
| Keywords: | cockchafers Bacillus thuringiensis PCR-RFLP cry8 |
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