| 2例马来西亚籍输入性基孔肯雅热病例的实验室诊断 |
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| 引用本文： | 洪烨,李小波,黄吉城,郑夔,师永霞,相大鹏,幸芦琴,郭波旋,李华,戴俊,钟玉清. 2例马来西亚籍输入性基孔肯雅热病例的实验室诊断[J]. 中国国境卫生检疫杂志, 2009, 0(2): 75-77 |
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| 作者姓名： | 洪烨 李小波 黄吉城 郑夔 师永霞 相大鹏 幸芦琴 郭波旋 李华 戴俊 钟玉清 |
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| 作者单位： | 广东出入境检验检疫局检验检疫技术中心;广东出入境检验检疫局卫生监管处; |
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| 基金项目： | 国家质量监督检验检疫总局科研基金项目(2008IK256) |
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| 摘 要： | [目的]通过系统的实验室检测发现并确认2例输入性基孔肯雅病例。[方法]采用实时荧光逆转录（Realtime RT-PCR）、逆转录PCR（RT—PCR）检测方法对病人血清进行检测，并对RT-PGR扩增产物进行核苷酸序列测定。[结果]通过实时荧光RT—PCR、RT—PCR 2种实验方法在该病例标本中检出基孔肯雅病毒核酸；对RT-PCR扩增产物进行测序，测出的521个碱基与基孔肯雅病毒核酸序列比对，结果同源性高达99％。[结论]2病例为输入性基孔肯雅实验室确诊病例。
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| 关 键 词： | 输入性 基孔肯雅热 实验室 诊断 |
Laboratory Diagnosis of Two Imported Chikungunya Disease Cases from Malaysia. |
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| Hong Ye,Li Xiaobo,Huang Jicheng,Zheng Kui,Shi Yongxia,Xiang Dapeng,Xing Luqin,Guo Boxuan,Li Hua,Dai Jun,Zhong Yuqing.. Laboratory Diagnosis of Two Imported Chikungunya Disease Cases from Malaysia.[J]. Chinese Journal of Frontier Health and Quarantine, 2009, 0(2): 75-77 |
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| Authors: | Hong Ye Li Xiaobo Huang Jicheng Zheng Kui Shi Yongxia Xiang Dapeng Xing Luqin Guo Boxuan Li Hua Dai Jun Zhong Yuqing. |
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| Affiliation: | 1. Inspection and Quarantine Technical Center;Guangdong Entry-exit Inspection and Quarantine Bureau;Guangzhou 510700;China;2. Division of Health Quarantine;Guangzhou 510623;China. |
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| Abstract: | Objective To find and confirm two imported Chikungunya disease cases in China by laboratory diagnosis. Method Using real time PCR and RT-PCR to detect the patient' s serum and sequencing the PCR product. Result Real time PCR and RT-PCR were positive for sample collected from the patient. Sequencing result showed that the PCR product (521bp) was Chikungunya virus gene segment. Conclusion Two cases were laboratory confirmed imported Chikungunya disease cases in China. |
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| Keywords: | Imported Chikungunya disease Laboratory Diagnosis |
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