| 尖吻蝮蛇毒抗凝血因子(ACF)含量和钙离子含量的定量测定及钙离子结合位点的初步研究 |
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| 引用本文： | 刘清亮,徐晓龙,余华明,王淳. 尖吻蝮蛇毒抗凝血因子(ACF)含量和钙离子含量的定量测定及钙离子结合位点的初步研究[J]. 无机化学学报, 1993, 0(1) |
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| 作者姓名： | 刘清亮 徐晓龙 余华明 王淳 |
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| 作者单位： | 中国科技大学应用化学系，中国科技大学应用化学系，中国科技大学应用化学系，中国科技大学生物系 合肥230026，合肥230026，合肥230026 |
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| 摘 要： | 由紫外光谱法测定蛋白质浓度,用ICP测定Ca含量,从而确定一个ACF分子中含一个钙离子.ACF中分子中除一个高亲和性钙结合位点外,至少还有一个低亲和性钙结合位点,只有当过量钙离子存在时,才可能在低亲和性钙结合位点上进一步结合钙离子.Tb~(3+)具有比Ca~(2+)离子更强的键合ACF能力,能定量结合在ACF中的两个位点上,且能全部取代ACF中Ca~(2+).文中提及的金属离子对ACF的抗凝血活性没有显著的影响.
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| 关 键 词： | 抗凝血因子 钙 铽 荧光 |
DETERMINATION OF THE CONTENTS OF THE Ca~(2+) ION IN ACF AND THE STUDY OF Ca~(2+)-BINDING SITES |
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| Liu Qingliang Xu Xiaolong Yu Huaming Wang Chun. DETERMINATION OF THE CONTENTS OF THE Ca~(2+) ION IN ACF AND THE STUDY OF Ca~(2+)-BINDING SITES[J]. Chinese Journal of Inorganic Chemistry, 1993, 0(1) |
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| Authors: | Liu Qingliang Xu Xiaolong Yu Huaming Wang Chun |
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| Abstract: | The corrective coefficient of the equation on determination of the concentration of ACF by UV spectral (280nm-260nm) has been introduced. Our studies show that each ACF molecule contains one of Ca2+ ion, and has two Ca2+-binding sites at least (stronger one and weaker others). Ca2+ ion can bind to the weaker site in ACF only in the condition of existence of excessive Ca2+ ion. Tb3+ ion is coordinated with ACF more strongly than Ca2+ ion, while Tb3+ ion can bind to ACF at both two sites quantitatively and replace of Ca2+ ion in ACF completely. All the metal ions mentioned in this paper do not give intensive effect on the anticoagulation activity of ACF. |
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| Keywords: | anticoagulation factor calcium terbium fluorescence |
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