不同防治方法对龋病患儿口腔链球菌数量的影响

目的探讨不同防治方法对龋病患儿口腔链球菌细胞数量的影响。方法随机抽样法选取400名学龄前儿童为研究对象,根据不同防治方法将其分为对照组(健康宣教,222名)与观察组(氟防龋,178名),跟踪随访1年,间隔6个月行口腔检查1次,比较2组龋病新增率,并于防治前、防治后6个月、12个月、24个月通过实时荧光定量PCR法测定各组无龋病儿童链球菌细菌数量。结果观察组末次随访龋病新增率显著高于对照组(P0.05);与防治前比较,2组无龋病儿童防治后6个月、12个月总链球菌、口腔链球菌数量均显著减少(P0.05);与对照组比较,观察组防治后6个月总链球菌、口腔链球菌、唾液链球菌、变异链球菌数量显著少,差异有统计学意义(P0.05);与对照组比较,观察组防治后24个月变异链球菌数量比较差异无统计学意义(P0.05)。结论健康宣教、氟化物使用均能有效预防龋病发生,减少口腔链球菌细菌数量,其中氟防龋效果更明显。

Effect of different control methods on number of Streptococcus oralis in children with caries

Objective To investigate the effect of different control methods on the number of Streptococcus oralis in children with caries.Methods Four hundred pre-schoolers were randomly selected as subjects and divided into the control group (health education,222 children) and the observation group (fluoride prevention of decayed teeth,178 children) according to different control methods.All the subjects were followed up for 1 year and received an oral examination every six months.The rate of new increase of caries was compared between the two groups,and the number of Streptococcocci in children without caries in both groups was determined by real-time quantitative PCR method before control and 6 months,12 months,24 months after control.Results At the end of the follow-up,the rate of new increase of caries in the observation group was significantly higher than that in control group (P＜0.05).The number of total Streptococcocci and number of Streptococcus oralis in children without caries in both groups were significantly lower at 6 months and 12 months after control (P＜0.05).Compared with the control group,the number of total Streptococcocci,Streptococcus oralis,Streptococcus salivarius and streptococcus mutans in the observation group was significantly smaller at 6 months after control (P＜0.05) but there was no significant difference in the number of Streptococcus mutans between the two group at 24 months after control (P＞0.05).Conclusion Both health education and use of fluoride prevention of decayed teeth can effectively prevent the occurrence of caries and reduce the number of Streptococcus oralis,but the effect of fluoride prevention of decayed teeth is better.