Gold nanoparticles disturb nuclear chromatin decondensation in mouse sperm in vitro |
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| Authors: | S T Zakhidov T L Marshak E A Malolina A Yu Kulibin IA Zelenina S M Pavluchenkova V M Rudoi O V Dement’eva S G Skuridin Yu M Evdokimov |
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| Affiliation: | 1.Biological Faculty,Lomonosov Moscow State University,Moscow,Russia;2.Koltzov Institute of Developmental Biology,Russian Academy Sciences,Moscow,Russia;3.Frumkin Institute of Physical Chemistry and Electrochemistry,Russian Academy Sciences,119991,Russia;4.Engelhardt Institute of Molecular Biology,Russian Academy Sciences,Moscow,Russia |
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| Abstract: | The effect of gold nanoparticles on mouse epididymal sperm has been studied using the model system of nuclear chromatin decondensation
in vitro. It is shown that the treatment of gametes, preliminary membrane-freed by sodium dodecyl sulfate, in the mediums
containing gold nanoparticles (with diameter ∼2.5 nm) in concentrations 1.0 × 1015 or 0.5 × 1015 particles/ml and following incubation in dithiothreitol solution (DTT) resulted in failure of chromatin decondensation process
and nucleus structure. We conclude that gold nanoparticles possess spermatotoxicity. The mechanism of cytotoxic effect of
gold nanoparticles may be related with their interaction with molecules of double-helix DNA. The model system studied in this
research is applicable for further investigations of cytotoxic effects of nanoparticles of different origin and made of different
metals. |
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