| 直接测序法与克隆测序法在单核苷酸多态性检测中的比较 |
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| 引用本文： | 张婷,单可人,李毅,何燕,齐晓岚,赵艳,谢渊,吴昌学,任锡麟,官志忠. 直接测序法与克隆测序法在单核苷酸多态性检测中的比较[J]. 贵阳医学院学报, 2006, 31(1): 18-20 |
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| 作者姓名： | 张婷 单可人 李毅 何燕 齐晓岚 赵艳 谢渊 吴昌学 任锡麟 官志忠 |
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| 作者单位： | 贵阳医学院,分子生物学重点实验室,贵州,贵阳,550004 |
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| 基金项目： | 贵州省科技厅科研项目 , 贵阳医学院校科研和教改项目 |
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| 摘 要： | 目的:了解PCR产物直接测序法与克隆测序法在单核苷酸多态性(SNP)检测上的差别.方法:对2型糖尿病患者的载脂蛋白J外显子2基因及旁侧进行聚合酶链反应(PCR)扩增,分别对其产物进行直接测序和克隆测序.结果:在样本的检测中, PCR产物直接测序法所测序列与43～60 bp以后克隆测序相同;检出一个突变位点并与GenBank( DQ012938)发表序列一致.结论:PCR 产物直接测序法和克隆测序法都是检测SNP的有效方法,但前者不仅特异性强,敏感度高,而且比克隆测序方法更为快速简便,节省材料.
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| 关 键 词： | 载脂蛋白类 多态性,单核苷酸 糖尿病,非胰岛素依赖型 聚合酶链反应 直接测序法 克隆测序法 |
| 文章编号： | 1000-2707(2006)01-0018-03 |
| 收稿时间： | 2005-11-22 |
| 修稿时间： | 2006-01-03 |
A Comparison of Direct Sequencing with Clone Sequencing in the Detection of Single Nucleotide Polymorphism |
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| ZHANG Ting,SHAN Ke-ren,LI Yi,HE Yan,QI Xiao-lan,ZHAO Yan,XIE Yuan,Wu Chang-xue,REN Xi-lin,GUAN Zhi-zhong. A Comparison of Direct Sequencing with Clone Sequencing in the Detection of Single Nucleotide Polymorphism[J]. Journal of Guiyang Medical College, 2006, 31(1): 18-20 |
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| Authors: | ZHANG Ting SHAN Ke-ren LI Yi HE Yan QI Xiao-lan ZHAO Yan XIE Yuan Wu Chang-xue REN Xi-lin GUAN Zhi-zhong |
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| Affiliation: | Molecular Biological Key Laboratory of Guiyang Medical College, Guiyang 550004, China |
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| Abstract: | Objective: To compare the sensitivity and accuracy of the 2 sequencing techniques for the detection of single nucleotide polymorphism(SNP).Methods: The exon 2 of Apo J gene was screened with polymerase chain reaction(PCR)in 8 patients with type 2 diabetes mellitus(DM).Samples were detected by direct sequencing and clone sequencing,and the results were compared.Results: One mutation was detected in exon 2 by two methods.Furthermore,it's same to the Homo sapiens apolipoprotein J(CLU) gene sequence in GenBank(DQ012938).Conclusion: The results show that the PCR direct sequencing is faster,easier,cheaper,and more practical than clone sequencing when used in SNP. |
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| Keywords: | apolipoprateins polymorphism, single nucleotide diabetes mellitus, non-insulin-dependent polymerase chain reaction direct sequencing clone sequencing |
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