不同尖孢镰刀菌对莲藕致病力差异与防治试剂筛选

为探明从不同病株分离的尖孢镰刀菌对莲藕致病力的差异,通过菌丝块接种的方式对莲藕发病情况进行测定,并选用3种化学药剂与2种生防菌剂进行室内平板抑菌试验。结果表明：从发病莲藕病部分离出的尖孢镰刀菌Fusarium commune对藕块和藕叶的致病力最强,分别达100.0%与83.3%;从甜瓜病株分离的尖孢镰刀菌F.oxysporum也可使藕块和藕叶发病,但发病率仅分别为66.7%与33.3%。化学药剂中,硫·酮多菌灵的防治效果最好,浓度为1.0 mg/L时,抑制率可达100.0%;生防菌剂中的哈茨木霉菌(3.0 g/L)与枯草芽孢杆菌(3.0 g/L)对尖孢镰刀菌F.commune的抑制率分别达78.8%、65.0%;下一步可进行大田防效验证。

Differences of Pathogenicity and Screening of Control Reagents of Different Fusarium oxysporum to Lotus Root

Abstract: In order to verify the differences of pathogenicity of Fusarium oxysporum, which isolated from diseased plants, the experiments were used to determine the incidence of lotus roots by inoculation of mycelium blocks. Then three kinds of chemical agents and two kinds of biocontrol agents were chosen for indoor plate antibacterial experiments. The results showed that the Fusarium commune isolated from the diseased part of the lotus root had the strongest pathogenicity for lotus root and lotus leaves, reaching 100.0% and 83.3%, respectively.F. oxysporum isolated from diseased melon could also cause the disease of lotus roots and lotus leaves, but the incidence rate was only 66.7% and 33.3%, respectively. Among the chemical agents, sulph-ketone sanmate had the best control effect with a concentration of 1.0 mg/L, the inhibition rate could reach 100.0%, the inhibition rates of trichoderma harzianum (3.0 g/L) and bacillus subtilis (3.0 g/L) in the biocontrol agent against Fusarium communecould reach 78.8% and 65.0%. The next step could be carried out to verify its control effect in the field.