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转基因棉对棉蚜生长发育及其主要代谢物质含量的影响
引用本文:薛丽,肖达,卢延,宋敦伦,高希武.转基因棉对棉蚜生长发育及其主要代谢物质含量的影响[J].华北农学报,2011,26(4):115-120.
作者姓名:薛丽  肖达  卢延  宋敦伦  高希武
作者单位:中国农业大学农学与生物技术学院昆虫系,北京,100193
基金项目:转基因生物新品种培育重大专项(2008ZX08012-04)
摘    要:近年来,转基因棉田非靶标害虫种群数量呈明显的上升趋势,棉蚜是转Bt(Bacillus thuringiensis)基因棉田发生危害较严重的非靶标刺吸性害虫之一.在实验室内,棉蚜分别用转Bt基因棉和亲本常规棉饲养40代以上,采用酶联免疫法( ELISA)检测转Bt基因棉SGK321上棉蚜体内的Bt蛋白含量,研究转Bt基因...

关 键 词:转Bt基因棉  非靶标害虫  棉蚜  代谢物质  ELISA

Effects of Transgenic Insect-resistant Cotton on Development and Metabolite Content of Cotton Aphid Aphis gossypii
XUE Li,XIAO Da,LU Yan,SONG Dun-lun,GAO Xi-wu.Effects of Transgenic Insect-resistant Cotton on Development and Metabolite Content of Cotton Aphid Aphis gossypii[J].Acta Agriculturae Boreali-Sinica,2011,26(4):115-120.
Authors:XUE Li  XIAO Da  LU Yan  SONG Dun-lun  GAO Xi-wu
Affiliation:XUE Li,XIAO Da,LU Yan,SONG Dun-lun,GAO Xi-wu(Department of Entomology,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China)
Abstract:In recent years,the population densities of non-target pests in transgenic Bt cotton fields were obviously increased.Cotton aphid Aphis gossypii(Homoptera: Aphidae) was one of the seriously non-target pests in transgenic Bt cotton field.One A.gossypii strain was respectively reared on transgenic Bt cotton line SGK321,expressing Cry1Ac insecticidal protein,and its transgenic parent cotton cultivar Shiyuan321 for more than forty generations in the laboratory.The contents of Bt protein,carbohydrate,lipid,prote...
Keywords:Transgenic Bt cotton  Non-target pest  Aphis gossypii  Metabolite  ELISA  
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