LOXL2及Snail蛋白表达水平与胆管癌患者术后生存率的关系

目的 探讨赖氨酰氧化酶样蛋白2(LOXL2)及Snail在胆管癌（CCA）组织中的表达及其与患者临床病理特征和术后生存预后的关系。方法 收集云南省第一人民医院2014年1月至2021年4月期间行手术切除的31例胆管癌病例及随访资料，应用免疫组织化学染色检测患者癌组织及癌旁正常组织标本中LOXL2及Snail的表达情况，分析2种蛋白表达与临床病理资料及预后的关系。结果 肿瘤组织中LOXL2及Snail蛋白表达量均明显高于癌旁组织，且LOXL2表达与Snail表达存在着一定的正相关关系（r=0.430,P=0.016）,Snail表达与淋巴结转移（P=0.025）有关，与LOXL2及Snail高表达患者比较，LOXL2及Snail低表达患者术后总生存率较高（P <0.05），单因素生存分析显示：Snail高表达、存在淋巴结转移与预后更差的胆管癌患者相关（P <0.05）。Cox多因素生存分析结果表明：淋巴结转移可能是影响胆管癌患者预后的独立因素（P=0.017）。结论 LOXL2、Snail在胆管癌组织中表达增加，LOXL2与Snail 2种蛋白的表达密切相关，Snail的过表...

Association between LOXL2 and Snail Expression Levels and Overall Survival Rate after Surgery in Patients with Cholangiocarcinoma

  Objective  To investigate the expression of lysoxygenase-like protein 2 （LOXL2） and Snail in the tissues of cholangiocarcinoma （CCA） and their association with clinicopathological features and postoperative survival.   Methods  31 patients with cholangiocarcinoma who underwent surgical removal in the First People’s Hospital of Yunnan Province from 2014 to 2021 and their follow-up data were collected. The expression of LOXL2 and Snail in cancer tissues and adjacent normal tissues of patients was detected by immunohistochemical staining, and the association between the expression of the two proteins and clinicopathological data and prognosis was analyzed.   Results  The expression levels of LOXL2 and Snail in tumor tissues were significantly higher than those in adjacent tissues, and there was a positive correlation between LOXL2 expression and Snail expression （r = 0.430, P = 0.016）. Snail expression was associated with lymph node metastasis （P = 0.025）. Compared with patients with high expression of LOXL2 and Snail, patients with low expression of LOXL2 and Snail had higher overall survival rate （all P < 0.05）. Univariate survival analysis showed that high expression of Snail and lymph node metastasis were associated with worse prognosis in patients with cholangiocarcinoma （all P < 0.05）. Multivariate Cox survival analysis showed that lymph node metastasis might be an independent prognostic factor for patients with cholangiocarcinoma （P = 0.017）.   Conclusion  The expression of LOXL2 and Snail is increased in cholangiocarcinoma tissues, and the expression of LOXL2 and Snail is closely related. The overexpression of Snail may be more prone to lymph node metastasis and promote the invasive behavior of tumor cells, and lymph node metastasis may be an independent factor affecting the prognosis of cholangiocarcinoma patients. Patients with LOXL2 and Snail overexpression have a relatively low postoperative survival rate.Therefore, the combined detection of LOXL2 and Snail is of great significance for the prognosis of patients with cholangiocarcinoma.