白细胞介素-4在脂多糖诱导急性肺损伤模型中的保护作用 |
| |
作者姓名: | 赵琨 肖云 杨纯 严志凌 董敏娜 向柄全 肖茗耀 |
| |
作者单位: | 1.昆明医科大学第三附属医院重症医学科 |
| |
基金项目: | 云南省科技厅-昆明医科大学应用基础联合专项基金资助项目(202001AY070001-081) |
| |
摘 要: | 目的 探讨白细胞介素-4(IL-4)在急性肺损伤(acute lung injury,ALI)中的保护作用。 方法 脂多糖(lipopolysaccharide,LPS)诱导A549细胞形成ALI细胞模型。使用不同浓度的IL-4(0.1 μg/mL、1 μg/mL、10 μg/mL)在不同时长下干预该模型,通过流式细胞术检测A549细胞凋亡率,ELISA法测定A549细胞分泌IL-1、IL-6、IL-10、TNF-α、TNF-γ情况。 结果 IL-4可降低ALI模型中A549细胞凋亡率、抑制Caspase3表达(P < 0.05),其效果随IL-4浓度增加及干预时长延长而加强;同时促进Bcl-2表达(P < 0.05),IL-4浓度1 μg/mL干预12 h时效果最佳。上述条件下,IL-4可降低ALI模型中A549细胞的IL-1、IL-6、TNF-α、TNF-γ,并增加IL-10(P < 0.05)。 结论 IL-4可以通过改善ALI体外模型中细胞因子分泌情况,在急性肺损伤中发挥正向调节作用。
|
关 键 词: | 急性肺损伤 白细胞介素-4 脂多糖 调节性T细胞 细胞因子 |
收稿时间: | 2022-03-28 |
Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models |
| |
Affiliation: | 1.Dept. of Critical Care Medicine2.Dept. of Gynecological Oncology,The 3rd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 6501183.Dept. of Emergency,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China |
| |
Abstract: | Objective To investigate the protective effect of interleukin-4 (IL-4) on acute lung injury (ALI). Methods ALI cell models were developed using lipopolysaccharide (LPS) to induce A549 cells. The model was treated with different concentrations of IL-4 (0.1 μg/mL, 1 μg/mL, 10 μg/mL) for different durations. Then the apoptosis rate of A549 cells was detected by flow cytometry, and the secretion of IL-1, IL-6, IL-10, TNF-α, TNF-γ of A549 cells was determined by ELISA. Results IL-4 reduced the apoptosis rate of A549 cells and inhibited the expression of Caspase3 in ALI model (P < 0.05). The effect was enhanced with the increase of IL-4 concentration and the extension of intervention time. IL-4 promoted the expression of Bcl-2 (P < 0.05), and the best effect was achieved when the concentration of IL-4 was 1 μg/mL for 12 h. Under the above conditions, IL-4 decreased IL-1, IL-6, TNF-α, TNF-γ and increased IL-10 in A549 cells in ALI model (P < 0.05). Conclusion IL-4 can positively regulate acute lung injury by improving cytokine secretion in ALI in vitro model. |
| |
Keywords: | |
|
| 点击此处可从《》浏览原始摘要信息 |
|
点击此处可从《》下载全文 |