反相离子对高效液相色谱法同时测定维生素B_1、B_2和菸酸含量

本文报告反相离子对高效液相色谱法(HPLC)同时测定强化混合蛋白质中的维生素B_1、B_2和菸酸。色谱柱为C_(18),流动相是庚烷磺酸盐液与甲醇(60∶40V/V),以紫外254nm检测。B_1、B_2和菸酸的最小检出量分别为15,5和6ng。样品分析CV％为4.71,2.6和5.86;回收率为92.21,90.25和92.33％,试样中其他成份对分析干扰很小,是一种筒单,快速,灵敏和精密度较好的测定方法,可作力强化食品和维生素制剂中B_1,B_2和菸酸分析用。

SIMULTANEOUS DETERMINATION OF VITAMIN B_1, B_2 AND NIACIN IN FORTIFIED MIXED PROTEIN BY PAIRED-ION REVERSED-PHASE HIGH PERFORMANCE CHROMATOGRAPHY

A high-performance liquid chromatographic procedure for the simultaneous determination of vitamin B1, B2 and niacin in fortified mixed protein was developed. The sample was heated on water bath by adding 0.1N HC1 at 100℃ for 30 min. Afterward, the vitamins in the filtrate of the sample were separated on a column of u-bondapak C18 with methanol/water containing 0.005M pic B7 ion pair reagents (40:60 V/V) as mobile phase, and then the eluate was measured at 254nm. The detection limits for vitamin B1, B2 and niacin were approximately 15 ng, 5ng and 6ng respectively. The average recoveries of vitamin B1, B2 and Niacin were 92.21,90.25 and 92.33% respectively. The coefficients of variation of these vitamins were all under 6%. The method is simple, rapid and accurate, so it could also be applied to the analysis of other samples such as fortified foods and multivitamin preprati-ons etc.